تفاصيل الوثيقة

نوع الوثيقة : مقال في مجلة دورية 
عنوان الوثيقة :
The Application of Multi-Colour Fluorescence In-situHybridisation to the Analysis of Abnormal Chromosomes inNon-Hodgkin's lymphomas
The Application of Multi-Colour Fluorescence In-situHybridisation to the Analysis of Abnormal Chromosomes inNon-Hodgkin's lymphomas
 
لغة الوثيقة : الانجليزية 
المستخلص : Numerical and structural chromosome aberrations are recognised as important diagnostic and prognostic indicators in human diseases, including non-Hodgkin's lymphomas (NHL). Recently, fluorescence insitu hybridization (FISH) has been used with increasing success to identify and characterise many chromosomal aberrations, leading to the development of multiple chromosome painting methods for simultaneous dctection of multiple probes in metaphase target cells. In this study, we have analysed 15 NHL biopsy samples and 5 human lymphoma cell lines (Daudi. HS602, Raji, MC116 and Namalwa). Some of the cell liiics were partially analysed cytogenetically in the early 1970s using solid staining techniques, but no chromosome analysis has been previously attempted on the HS602 cell line. The detection of chromosome abnoimalities in malignant lymphoma patients and cell lines by conventional cytogenetics is particularly problematic because of difficulty in routineIy preparing metaphases spreads of sufficient quality or quantity and the complex nature of many of chromosomal changes. We completed cytogenetic analyses on all of the NHL samples and the cell lines. We then followed a strategy of refining these preliminary findings by applying multicolour FISH with whole chromosome painting probes derived from flow sorted chromosome libraries, using direct and indirect combinatorial labelling with two fluorochromes, to detect up to five different chromosomes on the same target metaphase. The application of multiple chromosome painting has enabled complex rearrangements to be. characterised and made the identification of marker chromosomes possible 
ردمد : 1096-9896 
اسم الدورية : Journal of Pathology 
المجلد : 182 
العدد : 1 
سنة النشر : 1418 هـ
1997 م 
نوع المقالة : مقالة علمية 
تاريخ الاضافة على الموقع : Saturday, March 3, 2012 

الباحثون

اسم الباحث (عربي)اسم الباحث (انجليزي)نوع الباحثالمرتبة العلميةالبريد الالكتروني
محمد حسين القحطانيAl-Qahtani, Mohammed Hussainباحث رئيسيدكتوراهmhalqahtani@kau.edu.sa

الملفات

اسم الملفالنوعالوصف
 32545.pdf pdf 

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