Document Details
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Document Type |
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Thesis |
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Document Title |
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Detection of some Antibiotic-Resistant Bacterial Species and some of their Resistance Genes الكشف عن بعض أنواع البكتيريا المقاومة للمضادات الحيوية وبعض الجينات المسئولة عن تلك المقاومة |
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Subject |
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Faculty of Sciences |
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Document Language |
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Arabic |
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Abstract |
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Bacteria were the leading causes of the infectious diseases through the human history. This problem was expected to disappear by using antibiotics. The disproportionate use of antibiotics leads to the development of antibiotic resistance mechanisms in bacteria. Antibiotic resistance turn out to be one of our most serious health threats. Some pathogenic bacteria developed resistance to multiple classes of antibiotics. Therefore, the current study aimed to study the prevalence of this phenomenon. Disk-diffusion susceptibility test was carried out on seven Gram-positive (Bacillus cereus, Enterococcus faecalis, Micrococcus luteus, Staphylococcus aureus, Staphylococcus capitis, Staphylococcus haemolyticus and Staphylococcus saprophyticus and five Gram-negative (Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli, Escherichia coli normal, Salmonella Typhi and Shigella sonnei) species using 16 different antibiotics. Almost all bacterial strains used in the current study employed increased resistance to more antibiotics, in addition to high levels of antibiotic resistance compared to their previously mentioned resistance reports. They become multidrug resistant (MDR) strains, thus, they are referred to as “superbugs”. Molecular genetic analysis using Polymerase chain reaction (PCR) confirmed the presence of some of the tested antibiotic resistance genes, such as ampR, blashv-12, penA1, penA2, ampC1, ampC2, aac4A-cr, and vanR on plasmids, bacterial chromosomes or on both. |
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Supervisor |
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Prof. Dr. Sabah Mahmoud Mohamed Hassan |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1438 AH
2017 AD |
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Co-Supervisor |
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Dr. Abdulqader Mohamed Shaikh Omar |
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Added Date |
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Thursday, July 13, 2017 |
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Researchers
| مدى صائل الحارثي | Al-Harthi, Mada Sail | Researcher | Master | |
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