Document Details

Document Type : Thesis 
Document Title :
ENHANCEMENT PRODUCTION OF XANTHAN GUM FROM XANTHOMONAS CAMPESTRIS BY MOLECULAR GENETICS TECHNIQUES.
تعزيز انتاج صمغ الزانثان من الزانثزموناس كامبستريس بتقنيات الوراثة الجزيئية
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : Microorganisms have an important role in many industries Such as food and medical in addition to other industries as an example Xanthomonas campestris is bacteria that Produces xanthan gum although it is considered a threat that effects multiple plants and crops. xanthan gum is used in many industries such as the food industry in dairy products (low – non) fat, sauces and ice cream also its used in the oil industry and in cosmetics and water based paints. The global production value of xanthan gum is 400 million dollars. about 80,000 tons are Produced every year 20.000 tons are produced by Xanthomonas campestris Which is about 25% of the global production. This makes Studying Xanthomonas campestris Important and the different factors that affect its production and how to improve xanthan gum production. This study aimed to isolate and identify Local strains of Xanthomonas campestris and study different molecular Differences.in this study a total of 28 Xanthomonas campestris strains were Isolated from local Produced in Saudi Arabia . The distinctive properties of 16s rRNA gene as universal phylogenetic marker was employed to carry out comprehensive diversity study of the bacterial isolates in order to reveal the evolutionary and phylogenetic relationships among these isolates. A random amplified polymorphic DNA (RAPD) and Issr fingerprinting method have been used to fingerprint isolated 28 Xanthomonas campestris bacterial strains using seven different primers. The RAPD profiles revealed a high level of DNA sequence diversity. Selected strains of Xanthomonas campestris where Mutated with chemical (UV) and physical mutagenesis (Sodium Azide) To test Improving xanthan gum production levels. The result showed different responses between negative and positive effects. Production was improved up to 69.51% In comparison with original isolated strains. 
Supervisor : Prof. Salah El-Deen Mohamed Abo-Aba 
Thesis Type : Doctorate Thesis 
Publishing Year : 1441 AH
2020 AD 
Co-Supervisor : Prof. Jamal Sabir Mohamed Sabir 
Added Date : Wednesday, June 24, 2020 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
تركي مسعود الأحمديAL-Ahmadi, Torki MasoudResearcherDoctorate 

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