Document Details

Document Type : Thesis 
Document Title :
ISOLATION AND MOLECULAR CHARACTERIZATION OF POLYHYDROXYBUTYRATE (PHB) PRODUCING BACTERIA USING WASTE COOKING OIL AS A CARBON SOURCE.
العزل والتوصيف الجزيئي للبكتريا المنتجة للبولي هيدروكسي بيتا بإستخدام مخلفات زيت الطهي كمصدر للكربون
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : Disposal of petroleum based plastic is a threat to our environment and health since it is nondegradable. Polyhydroxybutyrate (PHB) is the best type of biodegradable polymer that accumulated by genera of bacteria. These bacterium use it as a carbon and energy sources. The properties of PHB are identical to the petroleum based plastics. The aim of this study was to isolate and characterized the best PHB producers that used waste cooking oil (WCO) as a carbon source. Twenty two bacterial isolates were obtained on nutrient agar media and modified C2M agar media from different soil samples, collected from Jeddah, Kingdom of Saudi Arabia. All WCO samples were collected from different domestic houses. All bacterial isolates used WCO as a carbon source. They were screened for PHB production by using Sudan Black B stain. Three bacterial isolates accumulated PHB and the quantity of PHB was determined by UV-VIS spectrophotometer. The bacterial isolate (NM17) is the best PHB producer which accumulate 50.10 % of the dry cell weight. The bacterial isolate (NM17) was identified based on morphological, physiological, biochemical tests and 16s rRNA gene sequence as Bacillus funiculus NM17. The best condition of PHB production were obtained using modified C2M broth media at pH.6.5, incubation temperature at 37℃ and incubation period was 2days. 
Supervisor : Dr. Mona Othman Albureikan 
Thesis Type : Master Thesis 
Publishing Year : 1442 AH
2020 AD 
Added Date : Monday, August 24, 2020 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
نوف عبد العزيز المنصورAlmansour, Nouf AbdulazizResearcherMaster 

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 46682.pdf pdf 

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