Document Details

Document Type : Thesis 
Document Title :
In vitro evaluation of the anti-tumor activity of bleomycin- loaded nanoemulsion
التقييم المعملي للنشاط المضاد للسرطان لبليومايسين المحمَل على مستحلب نانومتري
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : Loading the chemotherapeutic agents in nanoemulsions system has recently gained attraction in medicine due to their ability to improve the drug’s efficacy and reduce its adverse effects. The objective of the current study was to load bleomycin (BLM) in nanoemulsions (NEs) consisting of cinnamon oil and different buffer solutions using ultrasonication technique. The anticancer activities of the different (NEa, NEb and NEc) were evaluated in HeLa cervical cancer and SKOV-3 ovarian cancer cell lines. The cytotoxicity was examined by cell counting kit-8 (CCK-8) while the apoptosis was detected by flow cytometery and measurement of DNA fragmentation. The physical characteristics of the produced NEs were identified by the zetasizer. The higher cytotoxic NE formula in HeLa cells was 1BLM:2NEa, while 2BLM:1NEc exhibited the greatest potency in SKOV-3 cells. Therefore, they were selected in the next subsequent experiments. Results of the apoptotic evaluation revealed that BLM-loaded NE formulas were more apoptotic than free BLM in both tested cells. The DNA fragmentations of HeLa and SKOV-3 cells treated with BLM-loaded NE formulas were amplified by factor of (16.04 ± 0.14) and (7.64 ± 0.03) respectively, relative to all controls. The zeta average diameter and zeta potential of BLM-NEa were 524.33 ± 1.10 nm and 0.537 ± 0.002 mV, respectively whereas BLM-NEc has a mean z-average diameter and zeta potential of 522.57 ± 0.85 nm and -0.381 ± 0.003, respectively. The current study proved that loading BLM in NE based on cinnamon oil has considerably improved its efficacy as anticancer drug. 
Supervisor : Dr. Khadijah Saeed Balaamash 
Thesis Type : Master Thesis 
Publishing Year : 1442 AH
2021 AD 
Co-Supervisor : Dr. Sohair Mohammed Khojah 
Added Date : Sunday, August 15, 2021 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
روان سعد الغامديalghamdi, Rawan SaadResearcherMaster 

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