Document Details
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Document Type |
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Thesis |
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Document Title |
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The Effect of some Plant Extracts and Nano-compounds on Bacterial Quorum Sensing and Biofilm Formation on some Medical Devices تأثير بعض المستخلصات النباتية والمركبات المتناهية الصغر على التواصل البكتيري وتكوين البيوفيلم على بعض الأدوات الطبية |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Biofilm production causes serious issues in public health, medicine, and industry. Biofilms cause many chronic infections, multidrug resistance, and human deaths. Biofilm-associated infections require innovative biofilm inhibitors from various sources. The study's goal is to investigate bacterial biofilm formation and its effective factors, to discover safe antibacterial-antibiofilm agents from natural sources and nanomaterials that may be effective biofilm inhibitors, and to apply this control strategy on biofilm induced on some medical devices to prevent biofilm-associated chronic infections. Fourteen biofilm-forming bacterial isolates were quantified using Crystal violet staining, and the most biofilm-forming were chosen. Estimated exopolysaccharides, effective factors, and isolate comparisons were done. MICs were used to select the best plant extracts and nanoparticles for antibacterial-antibiofilm activity. The bacterial Quorum effect of specific materials Studying biofilm formation's sensing and motility. SEM and light microscopes assessed the selected materials' antibacterial and antibiofilm activities. After repeated manipulation by the selected materials, growth curve, antibiotic sensitivity, and biofilm formation were compared. Chromatography and cytotoxicity studies were done on selected plant extracts. Biofilm-forming isolates from medical devices were treated with chosen inhibitors. Acinetobacter baumannii, Listeria. monocytogenes, Serratia. marcescens, and Klebsiella. pneumoniae were selected with biofilm absorbance 0.64, 0.35, 0.54, and 0.44. L. Monocytogense had the highest EPS carbohydrate content at 0.21 ±0.02 μg/mg of cells. Temperature, medium, and pH affected exopolysaccharides. All isolates except K. pneumoniae were sensitive to organic-solved Arak (Ao), aqueous-solved Cranberry (Ca), and Pomegranate peel (Pa) extracts. Nanoparticles of ZnO, MgO, and MgZnO have the highest effects. Ca (12.5 mg/ml) and MgZnO (6.0 mg/ml) had the lowest MICs. Ca (25.0 mg/ml) and MgZnO (12.5) had the lowest BMICs. Ao and ZnO inhibited proliferation, EPS, biofilm, quorum sensing, and bacterial motility on K. pneumoniae. Cranberry was the only non-toxic item evaluated (25, 50, 100 mg/ml). When coated on medical device inner surfaces, all evaluated materials were strongly antibiofilm. Application of new natural approaches for inhibiting bacterial biofilm is important to prevent persistent and recurrent biofilm associated infections.
Keywords: Bacterial Quorum Sensing Inhibitors, Biofilm Formation, Exopolymeric Substance (EPS), Plant Extracts, Nanoparticles. |
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Supervisor |
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Prof. Dr. Magda Mohamm Ali |
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Thesis Type |
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Doctorate Thesis |
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Publishing Year |
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1445 AH
2023 AD |
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Co-Supervisor |
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Dr. Samyah Darwish Jastaniah |
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Added Date |
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Sunday, October 15, 2023 |
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Researchers
| تغريد ياسر جمال | Jamal, Taghreed Yasir | Researcher | Doctorate | |
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