Document Details

Document Type : Thesis 
Document Title :
Molecular Detection and typing of group A rotavirus among hospitalized children in Makkah
الكشف الجزيئي والتصنيفي للمجموعة أ لفيروس الروتا لدى الأطفال المنومين في مستشفيات مكة
 
Subject : Biochemistry department 
Document Language : Arabic 
Abstract : Group A Rotavirus is the leading cause of severe childhood diarrhea worldwide, resulting in ~ 600,000 deaths annually in children <5 years of age. Rotavirus infection is highly contagious and not easily controlled by improvements in hygiene and sanitation, as evidenced by similar incidence rates in developed and developing nations. The only control measure likely to have a significant impact on the incidence of severe disease is vaccination, but circulating rotaviruses are antigenically and genotypically diverse. The genotypic diversity of circulating rotavirus strains needs to be assessed before, during, and after vaccination of populations. Detection and genotyping of group A rotavirus strains from stool samples in young children with diarrhea in Makkah were examined using ElISA and genotyping by reverse transcription-nested multiplex PCR. Total Of 173 stool specimens, 75 samples was rotavirus positive. G1 type was the most common G-type (46.6%), followed by G2 (14.67%) ,G9 (6.6%), G3 (62.6%) G4 (1.3%) and G8 (1.3%). P[8] was the most common P-type (53.3%), followed by P[6] (14.6%) and P[4] (10.6%) .The prevalence of G-P combination type was genotype G1P[8] (46.6%), followed by G9P[8] (10.6%), G12P[8] (8%), G2P[6] (8%) G2P[4] (6%) G1P[6] (8%) G1P[4] (8%) G8P[8] (8%) G3P[8] (8%) G2P[8] (8%) G4P[8] (8%). 
Supervisor : Prof.Taha A. kumosani 
Thesis Type : Master Thesis 
Publishing Year : 1433 AH
2011 AD 
Co-Supervisor : Dr. Esam I. Azhar 
Added Date : Tuesday, December 13, 2011 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
نوره عبدالرؤوف القايديAL-kaiedi, Norah AbdulraoufResearcherMaster 

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