Document Details
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Thesis |
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Document Title |
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Taxonomic Studies on some species of genus Huernia R.Br. (Asclepiadaceae) growing in Wadi Thee Gazal in AL-Taif Province دراسات تصنيفيه لبعض أنواع جنس الهورنيا (الفصيله العشاريه) النامية في وادي ذي غزال بمحافظة الطائف |
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Subject |
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biological sciences department |
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Document Language |
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Arabic |
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Abstract |
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The genus Huernia R.Br. is a member of the family Asclepiadaceae . Huernia include about 70 species, is distributed in the tropical part of the world, South Africa and Saudi Arabia . About 8 species are growing in the western and southwestern regions of Saudi Arabia. Huernia species are so similar morphologically, that the confusion on their identification can be drown.Wadi Thee Gazal in Ash shafa region was the richest area with vegetation in Al-Taif province includes many species of genus Huernia.The aim of the present work is to do morphological , palynologycal and molecular studies of some Huernia species growing in wadi Thee Gazal. The studies include the morphological description of the Huernia species , the structure of pollinaria in addition to the DNA fingerprint using AFLP markers to perform detailed studies of polymorphism of 8 Huernia species under envestigation. As aresult of this research we were re-evaluate the taxonomic status of some species and re-instated at varietal level and subspecific level , 3 new species in addition to 2 new varieties and one subspecies are described ,the results of the morphological features of the studied species show the presence of many important characters, which can be used for separation Huernia species: H.saudi arabica, H.khalidbinsultanii var. A , H.khalidbinsultanii var. B , H.khalidbinsultanii ssp. C , Huernia sp.(collenette1176) , Huernia sp. nov. 1, Huernia sp.nov. 2 , Huernia sp.nov. 3 into two groups: The first group can be distinguish by the hairy like papillae which covered corolla inner surface,these group includes H.khalidbinsultanii var. A , H.khalidbinsultanii var. B , H.khalidbinsultanii ssp.C and Huernia sp.nov. 1, this group was divided into two sub-groups based on the corolla base color and lobes spreading , the first sub-gruop includes only Huernia sp.nov. 1 which characterized by yellow creamy corolla color with reflexed lobes, whereas the seconde sub-group has white creamy corolla color with steeply ascending lobes, this sub-groub includes the three rest, H.khalidbinsultanii ssp. C distinguish by larg flowers more than 3.5 cm in dim. and long pedicels > 10mm, whereas H.khalidbinsultanii var. A , H.khalidbinsultanii var. B has small flowers less than 3.5cm in dim. and short pedicels<10mm, we can differentiate between this two varieties by the triangular inner corona base in H.khalidbinsultanii var. A and straight one in H.khalidbinsultanii var. B.The second group includes H. saudi Arabica, Huernia sp.(collenette1176), Huernia sp.nov. 2 and Huernia sp.nov.3 which are characterized by conical papillae which covered corolla inner surface , this group divided into two sub-groups based on the multiblying of stem angles : the first sub-group can be distinguish by 5-angled stems which multiplying to 6-8 angles in the tip of some stems include Huernia sp.nov. 3 only ; whereas the second sub-group includes Huernia sp.(collenette1176)and Huernia sp.nov.2 and H. saudi arabica which are characterized by 5-angled stems without multiplication, this group divided into two sub-groups based on the wide of papillae base, H. saudi arabica distinguish by broad base papillae. Both Huernia sp.(collenette1176) , Huernia sp.nov. 2 were characterized by narrow base papillae, Huernia sp.nov. 2 distinguish by broad corolla tube and short intermediate lobes, whereas Huernia sp. (collenette1176)distinguish by narrow corolla tube and long intermediate lobes. Also the study of pollinaria structure of the investigated species showed the occurrence characters that can be used in the separation of studied species in to two groups : the first group consists of H.khalidbinsultanii var. A , H.khalidbinsultanii var. B, H.khalidbinsultanii ssp. C, Huernia sp.nov.1 which can be distinguish by the elliptic pollinia, this group divided into two sub-groups based on the length of germination crest , the first sub-group include of Huernia sp.nov. 1 only with germination crest at38 µm in length ,the second one includes H.khalidbinsultanii var. A, H.khalidbinsultanii var. B and H.khalidbinsultanii ssp. C with germination crest more than 38 µm in length , H.khalidbinsultanii ssp. C distinguish by increased length of corpasculum than its width , whereas both H.khalidbinsultanii var. A, H.khalidbinsultanii var. B was distinguish by equaling corpusculum lingth with its width, we can differentiate between this two species by the length of pollinium which are short in first species and long in the second one. The second sub-group consists of H. saudi arabica , Huernia sp.(collenette1176), Huernia sp.nov.2 Huernia sp.nov. 3 which can be distinguish by the oblong- elliptic pollinia, Huernia sp.nov.2 distinguish by broad caudical, Huernia sp.(collenette1176) distinguish by narrow groove corpasculum ,whereas both Huernia sp.nov. 3 , H. saudi arabica distinguish by broad groove corpasculum , we can separate between this two species by the width of corpasculum which can be ≤ 19 µm in H. saudi arabica and more than 19 µm in Huernia sp.nov. 3. AFLP analysis for the 6 species of Huernia using two primer combinations exhibited about 34.4% polymorphism with Combination I (EcoRI-ACG / Mse I– CAG) whereas about 21.87% polymorphism with Combination II (EcoRI- AGC / Mse I– CAG). Similarity matrix and dendrogram based on the data of AFLP-PCR analysis it is notice that the closest relationship was scored between H.khalidbinsultanii var.A, and H.khalidbinsultanii ssp. C, (80 %) followed by H. saudi arabica, and Huerinasp. nov. 3, (71%) then Huerina sp.nov.1, and Huerina sp.nov.2 (66 %) finally between H.khalidbinsultanii var. B, and Huerina sp.(collenette1176), (65%) .On the other hand, the lowest similarity value revealed between H.khalidbinsultanii var. A, and Huerina sp. nov. 1, also between Huerina sp. nov.3 and Huerina sp. nov. 2 (35 %) followed by H. saudi arabica, and H.khalidbinsultanii ssp. C,(36 %). On the other hand the dendrogram divided the plants into two main clusters, the first one divided into two sub-clusters, the first sub cluster included Huerina sp. nov.1, and Huerina sp. nov.2,while the second sub cluster comprised H.khalidbinsultanii var.A and H.khalidbinsultanii ssp. C .The second cluster divided into two sub cluster. The first one included H. saudi arabica, Huerina sp.nov. 3 but another one included Huerina sp.(collenette1176), H.khalidbinsultanii var.B .Two keys are drown, one based on the morphological characters, the second on the pollinaria characters…… |
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Supervisor |
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Dr.Nahed Waly |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1433 AH
2012 AD |
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Added Date |
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Wednesday, February 22, 2012 |
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Researchers
| سماح عبد الله الحربي | Alharbi, Samah Abdullah | Researcher | Master | |
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