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Document Type |
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Thesis |
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Document Title |
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Epidemiological and Molecular Characterization of Methicillin-Resistant Staphylococcus aureus (MRSA), among local clinical isolates التوصيف الوبائي و الجزيئي للمكور العنقودي الذهبي المقاوم للميثسيلين في العزلات المحلية |
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Subject |
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biological sciences department |
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Document Language |
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Arabic |
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Abstract |
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Methicillin–resistant Staphylococcus aureus (MRSA) has emerged as the most pervasive pathogen that causes severe morbidity and mortality worldwide.
The present study is concerned with epidemiological aspects, antimicrobial analysis, and molecular characterization. One hundred and one methicillin-resistant Staphylococcus aureus (MRSA) strains (out of 326 Staphylococcus aureus isolates collected from King Abdulaziz Hospital, between August 2009 and May 2011) were tested. The percentage of MRSA was 31% as compared to the total number of Staphylococcus aureus isolates. Nearly one-fifth (19.8%) of the isolates were mixed with other organisms. The mean age of MRSA patients was 39 years, and the age distribution in this study varied according to different units. The Intensive Care Unit (ICU) had the highest isolation rate: 18 (17.8%). Next was the Male Surgical (MS) unit with 14 (13.9%). The average duration (median) of hospital stay was 17 days. The highest isolation rates were from wound samples: 46 (45.5%), then from sputum: 14 (13.9%), and blood: 9 (8.9%). The acquisition modes of the MRSA strains isolated were categorized under: 20.8% community associated, 34.7% hospital associated, and the remaining 44.6% hospital-associated, community-onset. All isolates were sensitive to nitrofurantoin and vancomycin antibiotic. However, 80% were > 1 µg/ml, which indicates raised levels of MICs in vancomycin. Novel multiplex PCR for 16S, mecA, and Panton-Valentine leucocidine (PVL) was used; most of the PVL genes, i.e. 18 (39.1%), were detected in soft tissues and wound infections, which indicated the involvement of PVL gene with severe skin and soft tissue infections, especially necrotizing skin infections. MRSA SCCmec isolates were typed: The most predominant SCCmec among the examined isolates was Type V 43 (42.5%). |
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Supervisor |
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: Prof. Dr. Saleh Abdullah Ali Kabli |
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Thesis Type |
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Doctorate Thesis |
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Publishing Year |
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1433 AH
2012 AD |
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Added Date |
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Monday, May 14, 2012 |
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Researchers
| حسن عبدالله حمق | Hemeg, Hassan Abdullah | Researcher | Doctorate | |
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