Document Details
|
Document Type |
: |
Thesis |
|
Document Title |
: |
TAXONOMIC STUDIES OF EREMOPHILAGENUS (MYOPORACEAE) INTRODUCED IN SAUDI ARABIA دراسات تصنيفية لجنس الأريموفيلا (الميبورية) المستقدم للمملكة العربية السعودية |
|
Subject |
: |
biological sciences department |
|
Document Language |
: |
Arabic |
|
Abstract |
: |
Plant genus Eremophila belongs to family Myoporaceae which belongs to order Lamiales includes about 7 genus of 253 recognized species. These species were distributed throughout the dry and warm climates and consist of trees shrubs and under shrubs between 2 to 5 meters. Myoporaceae family spreads in Australia, South Pacific Islands and other areas including South Africa, Asia, Hawaii and the Western Indian Islands. Genus of Eremophila has played an important role in traditional medicine where many of them have been used in the medical and pharmaceutical purposes. Some species are valued as feed and grown as farm plants. There are currently 215 species recognized from the genus Eremophila. Majority of these species are endemic to the continent of Australia. However, it was possible to cultivate some of them in Kingdom of Saudi Arabia such as E. bignoniiflora, E. divaricata subsp. divaricata, E. glabra subsp. glabra, E. laanii, E. maculata subsp. maculata, E. oppositifolia subsp. oppositifolia, and E. pterocarpa subsp. pterocarpa. This research aims to study the morphological, anatomical characters, and DNA fingerprinting using Amplified Fragment Length Polymorphism (AFLP) technique for some selected species of the plant genus Eremophila cultivated in west of Saudi Arabia in Hada Al-Sham Research Station located in north of Jeddah city where there are no previous studies conducted in Saudi Arabia concerning these cultivated species. In addition, to our literature survey there was lack of such studies on these species in the Arabian Peninsula. Results of morphological studies, as character of spotted flowers, show that existence of two groups with spotted flowers including. E. bignoniiflora, E. divaricata subsp. divaricata, E. laanii, and E. maculata subsp. maculata. However, group of non-spotted flowers including E. glabra subsp. glabra and E. pterocarpa subsp. pterocarpa. Depending on character of fruits, they were ovoid and beaked in E. divaricata subsp. divaricata, E. laanii, and E. maculata subsp. maculata. They were ovoid and non-beaked in E. bignoniiflora, and E. glabra subsp. glabra. The species E. divaricata subsp. divaricata is characterized by the enclosed stamens in flowers. However, the other two species E. laanii and E. maculata subsp. maculata are characterized by the exerted stamen in flowers. Separation of two previous species was performed by using the number of ovules inside ovary. By studying shape and texture of fruit and form of leaves, distinguish between E. glabra subsp. glabra and E. pterocarpa subsp. pterocarpa was done. Studying the anatomical structures of the 7 species under investigation show variation in the mesophyll tissue in leaves were it was isolateral in species E. divaricata subsp. divaricata, E. glabra subsp. glabra, E. laanii, E. maculata subsp. maculata, and E. pterocarpa subsp. pterocarpa and isobilateral dorsiventral in species E. bignoniiflora and E. oppositifolia subsp. oppositifolia. The epidermal tissue of stem was simple in species E. laanii, E. maculata subsp. maculata, and E. pterocarpa subsp. pterocarpa and multiple in species E. divaricata subsp. divaricata and E. glabra subsp. glabra. In the anatomical structure of the stem of the seven species under investigation, axial parenchyma was banded with three cells in species E. laanii and vasicentric paratracheal in species E. maculata subsp. maculata and E. pterocarpa subsp. pterocarpa. Separation between previous species was performed by using character of type of epidermal cell of leaf. Also, according to the presence and absence of hairs on the outer surface of the leaf, E. glabra subsp. glabra is characterized by presence of non-branched glandular and non- glandular hairs while E. divaricata subsp. divaricata is characterized by its absence. Finally, we study genetic fingerprinting of the seven species of genus Eremophila in order to find the percentage of post-genetic by using indicator variation lengths of the pieces multiplier tests AFLP. Stages of the work included DNA isolation, purification, amplification, and detection the differences between the replicate segments of each selected plant. AFLP analysis illustrated the presence of 6 positive and 8 negative DNA markers that can be used to distinguish between the seven Eremophila species cultivated in Saudi Arabia. In addition, constructed phylogenetic tree divided the plants into three main clusters, the first one is divided into two sub-clusters, the first sub-cluster divided the plants into E. bignoniiflora, E. oppositifolia subsp. oppositifolia, and E. laanii, while the second sub-cluster have only one species E. pterocarpa subsp. pterocarpa. The second cluster is composed of two species E. divaricata subsp. divaricata and E. maculata subsp. maculata. Finally, the third cluster included only one species E. glabra subsp. glabra. |
|
Supervisor |
: |
Dr. Nahed Mourad Waly |
|
Thesis Type |
: |
Master Thesis |
|
Publishing Year |
: |
1433 AH
2012 AD |
|
Co-Supervisor |
: |
Dr. Sahar Rajab Elhadad |
|
Added Date |
: |
Monday, July 16, 2012 |
|
Researchers
| عبير صالح عطوة | Attwah, Abeer Saleh | Researcher | Master | |
|
Back To Researches Page
|