Document Details
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Document Type |
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Thesis |
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Document Title |
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Study of the Biophysical Properties of Magnetized Water and its Biological Effect on Human Erythrocytes دراسة الخواص الفيزيائية الحيوية للماء الممغنط وتأثيره الحيوي على خلايا الدم الحمراء البشرية |
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Subject |
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Biochemistry department |
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Document Language |
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Arabic |
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Abstract |
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The effect of static magnetic field on the biophysical and biochemical properties of different samples of water (tab, zamzam, bottled, distilled water), saline and erythrocytes suspension was investigated. In addition, the effect of magnetized saline on the erythrocytes antioxidant enzymes were evaluated.
Biophysical properties including pH, boiling point, rate of flow, conductivity and viscosity showed positive effect on applying magnetic field to the samples for different periods. Increasing the time of exposure increases the pH, boiling point, conductivity viscosity values and decreases the rate of flow, which may be due to increasing hydrogen bonding and water clusters. After withdrawal of water samples from magnetic field the measurements indicated that samples kept magnetization effect and tried gradually to return to their original values with time; decreasing the pH, boiling point, conductivity, viscosity values and increasing the rate of flow. Zamzam water had the highest value of pH, boiling point, conductivity and viscosity comparing with other samples, this is may be due to source and composition of this water, while the distilled water had the lowest value due to loss of minerals and salts during distillation process. The same results were obtained by applying magnetic field on the saline prepared by different methods. The biophysical properties of erythrocytes were studied for unmagnetized and magnetized suspensions. The results showed that by calculating the NaCl concentration (in the osmotic fragility test) which is able to make 50% hemolysis for the unmagnetized and magnetized erythrocytes suspension samples, it was found to be 0.5% and 0.45% respectively which means that, the pronounced shift towards lower concentration of NaCl for the magnetized sample indicates that the cell membrane became less permeable to water molecules and higher osmotic pressure is necessary to pump water molecules to the cells. This result indicates that the erythrocytes cell membrane became stronger to tolerate the osmotic pressure. The oxidative hemolysis of erythrocytes by ascorbic acid was also studied to confirm the result obtained from the osmotic fragility test ; it was found that the hemolysis rate for the unmagnetized sample was 0.0083 and that for magnetized sample was 0.0077, which means that the magnetized sample takes a longer time to complete hemolysis or can tolerate the oxidative stress more than the untreated sample. The activity of glucose 6-p dehydrogenase and antioxidant enzymes activities including glutathione peroxidase, glutathione reductase, were measured in erythrocytes before and after exposure to magnetic field. There was a significant increase in the enzymes activities in magnetized as compared with unmagnetized. The observations and findings described in this work are of great interest and importance, in a way that they help in applying magnetic treatment devices technology in various fields such as industry, medicine and agriculture to improve water properties. |
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Supervisor |
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Prof.Dr. Ibrahim Hassan Mohamed |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1433 AH
2012 AD |
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Co-Supervisor |
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Prof.Dr. Said Salama Moselhy |
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Added Date |
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Wednesday, October 10, 2012 |
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Researchers
| منال أحمد بن قبوس | Bin Gabous, Manal Ahmed | Researcher | Master | |
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