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Document Type |
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Thesis |
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Document Title |
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Molecular Genetic Typing of Avian Influenza Virus(H5N1) for Saudi Arabia Viral Strains تنميط وراثي جزيئي لفيروس انفلونزا الطيور(H5N1) للسلالات المتواجدة بالمملكة العربية السعودية |
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Subject |
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biological sciences department |
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Document Language |
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Arabic |
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Abstract |
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Periodically, a complete novel antigenic subtypes of influenza viruses have been introduced in the human population, causing large-scale global outbreaks with high death tolls. The most devastating influenza pandemic in modern recorded history, known as the “Spanish flu”, occurred in 1918–1919, killing up to 100 million people worldwide. In November 2007, Saudi Arabia’s Agriculture Ministry announced that it had culled 50,000 birds after a deadly H5N1 strain of bird flu was detected at a poultry farm in Al-Kharj, 150 kms south of Riyadh tests were carried out after 1,500 birds died in the farm, which had a total of 50,000 birds. As a consequence of all this, pandemic preparedness has become an important issue worldwide. This pandemic preparedness plans should include early recognition of novel influenza viruses and stock-piling of antivirals and candidate vaccines.Therefore, this study aimed at the development of a value-added molecular genetic approach for the diagnosis of H5N1 virus. This shall be done through the development of a Reverse Transcriptase –Polymerase Chain Reaction (RT-PCR) protocol to detect the Neuraminidase (N1) and Nucleoprotein (NP) genes specific for the H5N1 Saudis Arabian epidemic strains. This objective has been achieved through design oligonucleotide primers specific to the H5N1 KSA strain and establish a polymerase chain reaction protocols either in the classical or real time format in addition to test both specificity and sensitivity of the developed protocols. The results of this study showed that the designed oligonucleotide primers that are specific for the KSA H5N1 strains have a better sensitivity compared to the other primers that have been previously to detect a wide range of H5N1 strains. Therefore, these results recommends the implementation of the described protocols to detect the H5N1 KSA strains as a routine test. |
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Supervisor |
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Prof. Fahad A. Al - Fassi |
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Thesis Type |
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Doctorate Thesis |
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Publishing Year |
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1433 AH
2012 AD |
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Co-Supervisor |
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Prof. Aly Zain Elabidin Abdel salam |
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Added Date |
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Monday, December 31, 2012 |
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Researchers
| نجلاء سعود آل سعود | AAl - Saud, Najla Saud | Researcher | Doctorate | |
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