Document Details

Document Type : Thesis 
Document Title :
Molecular genetic diversity of Rhazya stricta Dence Plant in the makkah Al- mukarramah region
التنوع الوراثي الجزيئي لنبات الحرمل في منطقة مكة المكرمة
 
Subject : biological sciences department 
Document Language : Arabic 
Abstract : The present study aimed to studying some morphological characters and determining molecular markers to harmal plants (Rhazya stricta Dense) in the region of Mecca. Six locations in the western part of Tohamah plains, rich in Rhazya stricta (Decne) wild medicinal plant, were detected in this study. These locations were close and are on the same latitude (21) within the longitudes 40 and 39 from the east to the west. These locations are in a descending altitude of 1458 and 69 feet towards the west (the sea). Vegetative harmal (Rhazya stricta Dense) samples were collected from these locations. The Measurements of some morphological characters such as (mean leaf area, mean length of branches, mean length of the pods and mean of 100-seed weight. The maximum leaf area was achieved for plants collected from Al-Shemesi location, while the minimum was attained for plants collected from Al-Hada location. Harmal plants in Al-Shemesi and Al-Hada locations showed highest mean of length of branches, length of the pods and 100 seed weight compared to those in other locations under study. On the other hand, molecular biology techniques were used to determine the genetic variation at the molecular level (DNA). Two techniques were used, Random Amplified Polymorphic DNA-polymerase chain reaction (RAPD-PCR) and Inter-Simple Sequence Repeats-polymerase chain reaction (ISSR-PCR). RAPD-PCR data revealed the presence of 13 positive RAPD molecular markers in addition to only one negative molecular marker, while ISSR-PCR data revealed 9 positive and 8 negative molecular markers. RAPD and ISSR molecular techniques enabled us to detect polymorphic markers for Rhazya plants grown in different locations ranged from 16% in the Al-Shemesi and Al-Hada locations to 42% in Bahrah and Al-Karr. Studies for different Rhazya stricta (Decne) populations on the morphological and molecular levels indicated distinguishable genetic polymorphism among them. These results scope the light on the possible occurrence of genetic diversity in this plant among different locations enough to reconstruct the phylogenetic tree of this medicinal plant. A more extensive study of this plant in more locations in the kingdom might prove the occurrence of new subspecies or biotypes. This study also requires the use of more types of molecular markers that can be linked to the chemical composition and active ingredients with medical and pharmaceutical values in this important plant in folkloric medicine. 
Supervisor : Dr. Fotouh Mohamed El-Domyati 
Thesis Type : Master Thesis 
Publishing Year : 1434 AH
2013 AD 
Co-Supervisor : Dr .Mohammed Hamed Zainy Mutwakil 
Added Date : Monday, January 7, 2013 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
علي محمد أبو راسينabo rasain, Ali mohmmadResearcherMaster 

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