Document Details
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Document Type |
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Thesis |
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Document Title |
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Biochemical study on the toxic effect of melamine on rats دراسة كيميائية حيوية على التأثير السام للميلامين على الجرذان |
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Subject |
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Biochemistry department |
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Document Language |
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Arabic |
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Abstract |
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Due to the importance of melamine and its use in many products, the current study aimed to test the toxic effect of melamine supplementation for 28 days on male rats. 5 different doses of melamine were used in the diet; 5000, 10000, 15000, 20000 and 30000 ppm. The rats were divided into 6 groups and housed five for each group. The following investigations were tested; effect of melamine supplementation on the morphology and anatomy, complete blood count (CBC), serum electrolytes Na, K, Cl, Ca and P, kidney function (serum urea and serum creatinine), serum proteins (albumin, and total protein), serum bilirubin (total and direct bilirubin), serum liver enzymes (ALT, AST, ALP, GGT), serum melamine concentration, total body weight, food intake, FER, BWG%, food efficiency ratio, body weight gain and percentage of body weight gain, water consumption, SDS-PAGE of serum proteins, histopathological examination of seven organs (liver, heart, kidney, testis, ureter, urinary bladder and spleen). The melamine supplemented rats showed different degrees of hypertrophy and congestion according to the melamine dose, particularly the kidney and the ureter as a result of melamine toxicity. The CBC showed minimal changes in the treated groups. Na and Cl were decreased, whereas K, P and Ca were increased. Kidney function showed elevation of the mean values of serum creatinine and urea. Liver function enzymes were slightly affected. Serum protein was decreased and serum bilirubin was increased. The SDS-PAGE showed the induction of two new high molecular weight bands and another low molecular weight band as a result of melamine supplementation. The histopathological examination of the seven organs under study showed different pathological signs according to the dose used but the most injured one was the kidney. It could be said that in spite of the high toxic effect of melamine on kidney as revealed by the very high significant differences of the kidney functions, it did not greatly affect other organs. |
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Supervisor |
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Dr. Abdul Baset Al–Sieni |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1434 AH
2013 AD |
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Co-Supervisor |
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dr.Haddad Abdasameea |
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Added Date |
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Wednesday, January 16, 2013 |
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Researchers
| عبد الله أحمد معجمى | Majami, Abdullah Ahmed | Researcher | Master | |
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