Document Details
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Document Type |
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Thesis |
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Document Title |
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Study of prevalence of multidrug resistant Acinetobacter baumannii isolated from some patients of King Fahad General Hospital, Jeddah دراسة حوادث وانتشار بكتيريا الاسينيتوباكتر بومناي المعزولة من مستشفى الملك فهد العام بجدة |
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Subject |
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biological sciences department |
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Document Language |
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Arabic |
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Abstract |
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The prevalence of multi-drug resistant (MDR) Acinetobacter that is causing patient infections as well as their antimicrobial resistance patterns at the King Fahad General Hospital, Jeddah, Saudi Arabia was studied. 104 bacterial isolates were collected in six month period, from April to December 2010 from 102 patients with various specimens including sputum, wound, urine; blood; cerebrospinal fluid and other locations. Regardless of the specimen, there was a high rate of nosocomial MDR Acinetobacter spp. isolated from ICU. Of the 104 isolates of Acinetobacter spp., A. baumannii was (88.5%) and Non A. baumannii was (11.5%) by genotype. All of the strains were susceptible to colistin and higher MICs were recorded for all tested antibiotics except colistin (MIC50 ≤ 0.5 mg/l; MIC90 ≤1 mg/l). Twenty antibiotypes were observed among the Acinetobacter spp. The largest predominate antibiotype contained a total of 32 MDR strains including 29 A. baumannii (Ab) isolates 3 non A. baumannii isolates. Predominate antibiotype resistance for all the broad spectrum tested antimicrobial agents such as amikacin, cefepime, cefotaxime, ceftazidime, ciprofloxacin, gentamicin, imipenem, meropenem, piperacillin/ tazobactam, trimethoprim/ sulfamethoxazole excepted colistin and tigecycline. Respiratory tract specimens (sputum) represented nearly 72.2% of all the specimens collected in the ICU/CCU/SICU. Acinetobacter spp. was isolated from various types of infection, specially respiratory tract infection (27.8%), followed by urinary tract infections (20.8%), Acinetobacter ventilator-associated pneumonia (VAP) (11.1%), blood stream infections, burn wound infection and surgical site infection (9.7%), skin & soft tissues infection (6.9%), meningitis (1.4%) and other infection (6.2%.(
A multiplex PCR was used to identify antibiotic resistance genes in Acinetobacter spp. Out of 104 DNA extracts received, 92 were positive for OXA-51-like gene but 79 isolates were positive for OXA-23 gene, 3 isolates of A. baumannii were positive for OXA-24 gene and none were positive for OXA-58. The OXA-51 gene was found in both resistant and susceptible isolates, and was not associated directly with carbapenem resistance. The RAPD fingerprints of A. baumannii isolates were typed into three main clusters (n=15, n=41 and n=12). This study help taking effective measures for controlling Acinetobacter and data could be used in future as medical reference. |
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Supervisor |
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Dr. Saad Barki Al Masoudi |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1434 AH
2013 AD |
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Co-Supervisor |
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Dr. Magda Mohamed Aly |
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Added Date |
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Monday, March 18, 2013 |
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Researchers
| نهى قايد الحميدي | AL humidi, Nuha Qaid | Researcher | Master | |
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