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Document Type |
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Thesis |
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Document Title |
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Polymorphisms of the Duffy Antigen Receptor for Chemokines (DARC) Gene and its Relation to Osteoporosis in Saudi Postmenopausal Women الأشكال المتعددة لجين مستقبل مولد المضاد دافي للكيموكينات وعلاقته بهشاشة العظام لدى السيدات السعوديات ما بعد انقطاع الطمث |
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Subject |
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Biochemistry department |
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Document Language |
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Arabic |
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Abstract |
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Osteoporosis defined as a reduction in bone mass and disruption of bone architecture which will lead to increase in bone fragility and fracture risk. This disease specially targeting postmenopausal women, 67% of postmenopausal Saudi women suffering from osteoporosis. Low bone mineral density (BMD), gender, genetics and other risk factors could participates in the occurrence of osteoporosis. Considering this, several researches have studied the role of many genes in susceptibility of developing this disease such as vitamin D receptor and estrogen receptor alpha. Also, genetic studies on mice had pointed to region on chromosome 1 containing a gene responsible for BMD regulation through controlling osteoclast formation called Duffy antigen receptor for chemokine (DARC). This study focused the effort to evaluate the association between polymorphic FY*A, FY*B and FY*BGATA-1 alleles of DARC gene and osteoporosis risk in postmenopausal Saudi women. To the best of our knowledge, this is the first report of an attempted to see an association between the DARC and osteoporosis in human.
This study included 80 volunteers, living in Jeddah, Saudi Arabia, with an age range 45-85 year old, that were divided into groups according to bone mineral density (BMD) (T-score) at two sites (lumbar spine and femoral neck) as determined by DXA. Measuring BMD at lumbar spine, subjects were classified as 30 with osteoporosis, 21 with osteopenia and 29 were normal. Measuring BMD at the femoral neck, 11 had osteoporosis, 33 had osteopenia and 36 had normal BMD. Genotypes were determined for all subjects using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). Contingency analysis was performed for risk estimation.
In the whole study group, FY*B allele and FY*B/FY*B genotype showed the highest frequency with 75.63% and 62.5%, respectively. Remarkably, mutated C allele (FY*BGATA-1) were shown a higher frequency in the control group than the other groups and women carriers of CC genotype were shown a higher BMD at lumbar spine and femoral neck than women with other genotypes. No significant differences was found between DARC gene polymorphisms and the study groups. In conclusion, it is very interesting and unique that the FY*B allele occurrence is predominant in the Saudi women subjects. |
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Supervisor |
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Prof. Dr. Taha A. Kumosani |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1434 AH
2013 AD |
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Co-Supervisor |
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Dr. Archana P. lyer |
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Added Date |
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Saturday, June 1, 2013 |
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Researchers
| آلاء باسم التاجي | Taji, Alaa Basim | Researcher | Master | |
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