Document Details

Document Type : Thesis 
Document Title :
Intracellular Signaling of N-Formyl-Methionyl-Leucyl-Phenylalanine in Human Peripheral Blood Neutrophils
الإشارات الخلوية الداخلية للإن فورمايل ميثونايل ليوسايل فينايل ألانين في خلايا دم الإنسان المتعادلة الأصباغ
 
Subject : Biochemistry department 
Document Language : Arabic 
Abstract : Human polymorphonuclear neutrophils (PMN) play a critical role in host defences against invading microorganisms, and, in response to a variety of stimuli. They are a major source of reactive oxygen species (ROS) which are essential for bacterial killing and may induce oxidative stress in tissues. A precise regulation of the oxidase activity is therefore necessary. The respiratory burst is one of the marked responses of neutrophils for stimulation of their chemokine receptors by a chemotactic peptide, such as N-formylmethionylleucylphenylalanine (fMLP). The intracellular signals arising from the fMLP receptors have been proposed to be mediated by heterotrimeric G-protein and phosphoinositide 3-kinase (PI3K), eventually leading to NADPH oxidase activation. fMLP-induced respiratory burst is is accompanied by PI3K activation. In the present study, pharmacologic inhibition of PI-3Kγ activity blocks neutrophil respiratory burst activity stimulated by fMLP. PI3Kγ was shown to play an important role in signal transduction in fMLP-induced respiratory burst in human neutrophils. The aim of this study was to determine the effect of a selective PI3Kγ inhibitor (PI3Kγ inhibitor II) on the superoxide anion production induced by fMLP in human peripheral blood neutrophils collected from healthy donors. The respiratory assay was used to find out the optimal concentration of fMLP with the superoxide dismutase (SOD) as an inhibitor to the reduction of ferricytochrome C with PI3Kγ. By using the PI3Kγ inhibitor II to find out if fMLP activates the release of superoxide anion from neutrophils via a PI3Kγ pathway, or partially dependent on PI3Kγ with another pathway. The results showed that the optimal concentration of fMLP that induced the greatest superoxide production was 100 nM and the optimal concentration of PI3Kɣ that inhibits superoxide anion was 1 nM. It is recommended that additional experiments be conducted using more blood samples and different concentrations of fMLP. 
Supervisor : Dr. Mohamed Farouk Elshal 
Thesis Type : Master Thesis 
Publishing Year : 1434 AH
2013 AD 
Co-Supervisor : Dr. Sawsan Hassan Mahassni 
Added Date : Tuesday, June 18, 2013 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
حنان حامد العويضيAl-Owaidhy, Hanan HamidResearcherMaster 

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