Document Details
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Document Type |
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Thesis |
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Document Title |
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In vitro Evaluation of Simvastatin Microemulsion Formula in Liver Cancer Cells التقييم المعملي للسيمفاستاتين المحمل على مستحلب مايكرومتري في خلايا الكبد السرطانية |
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Subject |
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Biochemistry department |
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Document Language |
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Arabic |
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Abstract |
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Hepatocellular carcinoma is one of the major health concerns worldwide. Simvastatin, a cholesterol-lowering drug, displays numerous pleiotropic effects including anti-cancer activity. In this study, simvastatin was encapsulated in a microemulsion, which is an isotropic mixture of oil, water, surfactant and cosurfactant, in order to be evaluated as an anticancer drug against hepatocellular carcinomas. A pseudo ternary phase diagram, produced by mixing afixed weight ratio of 2:1 of Cremophor EL (surfactant)-to- Transcutol (cosurfactant), Captex 355 (oil) and distilled water, was constructed to find out the region of the one-phase microemulsion. The weight percentages of the selected formula for loading simvastatin consisted of 36.9% of Cremophore EL/Transcutol, 29% of Captex 355 and 34% of distilled water. The shape of both the blank microemulsion (Blank-ME) and freshly prepared simvastatin loaded-microemulsion (Fresh D-ME) droplets, revealed by scanning electron microscopy, was spherical. The antitumor activity of both Blank-ME and Fresh D-ME plus the Old D-ME, a simvastatin loaded-microemulsion kept for 2 weeks included to assess the stability of the combination formula, were examined in HepG2 liver cancer cells and compared with A549 non-small cell lung cancer, HCT 116 colon cancer, MCF-7 breast cancer and HFS human foreskin cells. The assessment involved the sulphorhodamine B (SRB) assay, ApopNexin FITC detection kit, the light microscope and hemolysis assay. It has been found that all of the microemulsion formulations of the simvastatin had stronger antitumor effect against all of the selected cancer cells (HepG2, A549, HCT 116 and MCF-7) than Zocor. Microemulsion formulations reduced the cytotoxicity of simvastatin administered unto HFS and red blood cells. In conclusion, microemulsion formulation improved the anticancer effect of simvastatin and reduced its side effect. |
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Supervisor |
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Dr. Mayson Husni Alkhatib |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1434 AH
2013 AD |
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Co-Supervisor |
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Dr. Wadiah Saleh Backer |
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Added Date |
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Sunday, June 23, 2013 |
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Researchers
| سحر سلطان الميرابي | AL-Merabi, Sahar Sultan | Researcher | Master | |
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