Document Details

Document Type : Thesis 
Document Title :
Prevalence Study Of Cytomegalovirus (CMV) Infection In Some Post Kidney Transplant Patients
دراسة انتشار الإصابة بالفيروس المضخم للخلايا (CMV) في بعض مرضى زراعة الكلى
 
Subject : biological sciences department 
Document Language : Arabic 
Abstract : Human cytomegalovirus (HCMV) is a common virus that infects most people at sometime during their lives either cause illness or not. CMV is a member of the beta herpes virus which can spread from person to person by direct contact and the body fluid. It can also be transmitted from the infected mother to her fetus or newborn and by solid organ Transplantation. Cytomegalovirus (CMV) infections are among the most common infections that occur following solid organ transplantation. CMV disease ranges from asymptomatic viremia to CMV syndrome and to more serious tissue-invasive disease. As CMV prophylaxis, treatment, diagnostics, and overall awareness have improved, the general trend has been towards earlier diagnosis of CMV disease and more mild clinical presentations in solid organ transplant recipients. Several diagnostic tests are available to detect CMV which include serological tests and more specific PCR techniques.Our study was conducted to determine the prevalence of HCMV in a sample of the eighty six patients who had post renal transplantation (more than six months). Data was collected using blood samples from patients followed up in King Abdul-Aziz Medical City – Western Region. Serological tests for Immunoglobulin M (IgM) and Immunoglobulin G (IgG) were done for CMV in all samples using ELISA technique.All samples 86 (100%) were IgG positive whereas only 31 (36%) were found to be IgM positive. In addition, samples were tested for detection of CMV DNA by Real Time PCR which showed that only 6 (7%) samples were PCR positive. Statistical analysis showed that there is significant difference in the seropositivity result between the IgM and PCR (p<0.05). Additionally, our study revealed that the prevalence of cytomegalovirus was significantly higher in females (50%) as compared to males (30 %) by IgM. The HCMV IgG results indicate a high prevalence of either past HCMV infection or exposure in the tested group, while the finding of IgM may reflect a recent infection or reactivation. HCMV detection by IgM in our study indicated a high prevalence among renal transplant patients, it was found that the actual prevalence is low if PCR was used. In conclusion, serological tests using ELISA are not diagnostic for detection of CMV and PCR should be used as a confirmatory test. We advocate use of PCR in screening post transplant patients for earlier diagnosis and treatment. Our study indicated higher prevalence of CMV among females which need to be confirmed by other studies from different centers in the Kingdom. 
Supervisor : dr. Nazar A. M. Redwan 
Thesis Type : Master Thesis 
Publishing Year : 1434 AH
2013 AD 
Added Date : Monday, June 24, 2013 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
نشــاط جــزاء العتيبيAl-Otaibi, Nashat JazaaResearcherMaster 

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