Deanship of Graduate Studies | Researches | Early Detection and Molecular Diagnosis of Some Most Common Bacterial Genus in Cystic Fibrosis Patients in Jeddah

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Deanship of Graduate Studies

Document Details

Document Type	:	Thesis
Document Title	:	Early Detection and Molecular Diagnosis of Some Most Common Bacterial Genus in Cystic Fibrosis Patients in Jeddah الكشف المبكر والتشخيص الجزيئي لبعض اجناس البكتيريا الأكثر شيوعا في مرضى التليف الكيسي بجدة
Subject	:	biological sciences department
Document Language	:	Arabic
Abstract	:	Progressive loss of lung function resulting from the inflammatory response to bacterial colonization is the leading cause of mortality in cystic fibrosis (CF) patients. A greater understanding of these bacterial infections is needed to improve lung disease management. This research was aimed to isolate and identify bacteria associated with CF patients by using molecuolar techniques. we used Polymerase Chain Reaction- Restriction Fragment Length Polymorphism profiling (PCR-RFLP) and Plasmid profiling to identify and characterize, with prior cultivation, 25 bacterial isolates from CF patients were compared with 24 isolates from non CF patients. Four different species were isolated in this study; P.aeruginosa, S.aureus, K. pneumoniae and A.baumannii. Same RFLP bands were found in all P.aeruginosa isolates, S.aureus and K. pneumoniae isolates had the same bands. Different genotypes were found in A.baumannii isolates. Plasmids were detected in 9 (39%) P.aeruginosa isolates,7 (53.8%) A.baumannii isolates, 5 (100 %) K.pneumoniae and 4 (50%) S.aureus isolates. In conclusion, our results show that CF patients in Jeddah had un common species in CF patients also the PCR-RFLP technique has High sensitivity for identifying species associated with infection in CF patients. Different numbers and sizes of Plasmids were detected in the isolates and the appearance of the plasmid in the isolates was vary from Species to another.
Supervisor	:	Dr. Ahmed Mahmoud Al-Hejin
Thesis Type	:	Master Thesis
Publishing Year	:	1434 AH 2013 AD
Co-Supervisor	:	Dr. Salah Eldeen Abo Aba
Added Date	:	Sunday, July 14, 2013

Researchers

Researcher Name (Arabic)	Researcher Name (English)	Researcher Type	Dr Grade	Email
خديجة محمد بن محفوظ	Bin Mahfouz, Khadijah Mohammed	Researcher	Master

Files

File Name	Type	Description
35720.pdf	pdf

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