Document Details
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Document Type |
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Thesis |
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Document Title |
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Physiological and Histological Studies for the toxic changes induced by Fenvalerate on rats and protective role of Vitamin C دراسات فسيولوجية ونسيجية للتغيرات السامة التى يسببها الفينفاليريت في الجرذان والدور الوقائي لفيتامين ج |
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Subject |
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biological sciences department |
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Document Language |
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Arabic |
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Abstract |
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This study aimed to study the protective role of vitamin C against the toxic effects of the fenvalerate insecticide in rats. The study include the changes in body organs weights and enzymes of blood , plasma , the liver and kidney, protein content, and in the variations of the enzymes AST, ALT, ACP, ALP, LDH and ACHE in the plasma, the liver and the kidney. Also the study demonstrate the variations in the concentrations of the TBARS substance and the antioxidant enzymes such as SOD, GST, GSH, and SH in the plasma, liver and kidney, in addition to tissue variations in liver kidney and testis using the Light microscope. The experiment extended for 30 days, and the animals were divided into 6 group; Group 1: Control group fed on corn oil. Group 2 (HD): fed 8.2 mg/kg fenvalerate, Group 3 (LD): fed 0.82 mg/kg fenvalerate, Group 4 (Vit. C): fed on 200mg/kg Vitamin C, Group 5 (HD+Vitamin C): fed on 200mg/kg Vitamin C and 8.2 mg/kg fenvalerate, Group 6 (LD+Vitamin C): fed 200 mg/kg Vitamin C and 0.82 mg/kg fenvalerate. The results indicated a significant reduction in body weights at the end of the experimental groups compared to the initial state and between the HD and LD fenvalerate groups and the control one, with significant increase in body weight of the groups HD+VC and LD+VC, and the liver and testis weights, and no significant difference in the kidney weight in HD and LD groups, with improvement in HD+VC and LD+VC groups but no change took place in VC group. Protein content was significantly reduced in liver and kidney in HD and LD – fenvalerate groups with significant increase in HD+VC and LD+VC groups, but no change occurred in the VC group. There was significant difference in the number of red blood cells and white blood cells (Leukocytes), and hemoglobin and hematocrit in HD and LD groups, with improvement in blood characters in HD+VC and LD+VC groups, but no change in VC group compared to control one. There was significant reduction in liver protein and globulin, and significant increase in albumin/globulin ratio in HD and LD groups, with improvement in the group HD+VC and LD+VC groups. The results also showed significant increase in glucose and bilirubin level in plasma in HD and LD groups and after addition of vitamin C there was significant reduction in HD+VC and LD+VC groups. There was significant difference in the plasma Lipoproteins, TL, Cho, TG, HDL, LDL and VLDL in HD and LD groups, with improvement after the addition of vitamin C to the fenvalerate. There were significant differences in the enzyme activities such as AST, ALT, ACP, ALP, LDH and ACHE in plasma, liver and kidney in HD and LD groups, with improvement in there enzyme concentrations in HD+VC and LD+VC groups compared to control, with no change in VC group. Also, significant difference occurred in the activities and concentrations of TBARS, SOD, GST, GSH and SH group in plasma, liver and kidney in HD and LD group, with improvement in HD+VC and LD+VC groups, but no change in VC group. The Light microscopic examination showed hepatocyte degeneration with cytoplasmic and nuclei infiltration with sinusoidal curves squeeze in HD and LD groups, but the hepatocytes were normal in HD+VC and LD+VC groups, and no change in VC group. The renal tissues of HD and LD groups showed certain degenerated uriniferous tubules and dilatation of Bowman's capsule while the renal tissues pretreated with vitamin C in HD+VC and LD+VC groups, and no change in VC group. Fenvalerate-treated groups showed the pathologic effects in which the structures of the seminiferous tubules were severely damaged, and the thickness of the tubular walls was reduced. The number of germinal cells was greatly decreased with a disturbance in their diameter. Low dose fenvalerate and vitamin C rat group showing sustainable normal but irregular seminiferous tubules with large intercellular space, but high dose and vitamin-treated group showing decrease pathologic effects in which the structures of the seminiferous tubules were slightly damaged with bleeding in intercellular space and no change in VC group. The study revealed that fenvalerate has a destructive effect on the structure and functions of the tissues and organs of rats, by increasing the free radicals, whose effect was reduced by using vitamin C as an antioxidant. |
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Supervisor |
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Prof. Hussein Khamis Hussein Ali |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1434 AH
2013 AD |
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Co-Supervisor |
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Dr. Moustafa Hussein Roshdy Elnaggar |
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Added Date |
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Tuesday, October 1, 2013 |
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Researchers
| جميل محمد الديلمى | Al Dailamy, Jamil Mohammed | Researcher | Master | |
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