Document Details

Document Type : Thesis 
Document Title :
In vitro Evaluation of Docetaxel Loaded on Microemulsion Formula in Breast and Non-Small Lung Cancer Cells
التقييم المعملي للدوسيتاكسال المحمل على مستحلب مايكرو متري في خلايا سرطان الثدي والرئه غير المصغره
 
Subject : Biochemistry department 
Document Language : Arabic 
Abstract : Docetaxel, an anti-mitotic chemotherapeutic agent belonging to the second generation of the taxoid family, is mainly used for the treatment of various cancers. The higher concentration of Tween 80, used in docetaxel formulation marketed as Taxotere, has been found to have severe side effects. In this study, docetaxel was loaded on a microemulsion formula, consisted of weight percentages of 37 of cremophor/transcutol mixture (2:1, w/w), 29 of capryol 90 and 34 of water. It was characterized using scanning electron microscope (SEM) and its antitumor activity was evaluated in vitro against A549 non-small cell lung cancer and MCF-7 breast cancer cells. In order to assess the efficacy of the docetaxel-loaded-microemulsion, it was subjected unto other cancer cells, which were HCT116 colon cancer and HepG2 liver cancer. The safe effect of the drug-loaded formulas was examined by administering them into HFS human foreskin cells. The antitumor activity study involved screening of cytotoxicity using sulphorhodamin B (SRB) assay, determination of hemolysis activity, and studying the mechanism of cell death by detecting the apoptosis using ApopNexin FITC detection kit and viewing the treated cells under the light microscope. The mean diameter of the blank microemulsion droplet, determined by SEM, was 89.32 ± 25.49, while the microemulsion formula loaded with docetaxel, have increased significantly to 205.3 ± 13.10. It has been found that 5µM of docetaxel-loaded- microemulsion was more cytotoxic than Taxotere when subjected unto A549 cells, while a 1 µM of docetaxel-loaded- microemulsion, applied into MCF-7 cells, had toxicity more than Taxotere. The effects of the docetaxel-loaded- microemulsion on the HFS cells were safe. In conclusion, formulating the docetaxel in a microemulsion formula improved the efficiency of the drug besides reducing its side effect. 
Supervisor : Dr. Mayson Husni Alkhatib 
Thesis Type : Master Thesis 
Publishing Year : 1434 AH
2013 AD 
Co-Supervisor : Dr. Sawsan Hassan Mahassni 
Added Date : Monday, October 7, 2013 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
وفاء عبدالغفار القايديAl-qaidi, Wafaa AbdulgafarResearcherMaster 

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