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Document Type |
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Thesis |
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Document Title |
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Modulation of antioxidant enzymes of rat kidneys exposed to food additives by bees honey تعديل مستويات الإنزيمات المضادة للأكسدة في كلى الجرذان المعرضة للإضافات الغذائية باستخدام عسل النحل |
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Subject |
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Biochemistry department |
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Document Language |
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Arabic |
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Abstract |
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Food additives have been used for flavoring, coloring and extension of the useful shelf life of food, as well as the promotion of food safety. Recently the use of food additives was increased and the levels of human exposure to such agents are very broad, thus feeding over long periods may continually possess potential hazards to the human health. Therefore, the aim of this work was to evaluate the protective role of bees honey against the adverse effects on the kidney functions induced by food additives (sodium nitrite as a food preservative and metanil yellow as food colorants) in male albino rats. The results of this study revealed that the oral administration of sodium nitrite (10 mg/ Kg body weight) for 8 weeks induced renal dysfunction as reflected by a significant elevation in serum levels of uric acid and creatinine and significant decrease in serum level of urea, while non-significant in serum urea as well as significant increase in serum creatinine and uric acid after treatment with metanil yellow (50 mg/ Kg) for 8 weeks. These results are apparently linked to the presently observed decrease in sodium level and increase in potassium in serum after treatment with sodium nitrite while increased of serum sodium and serum potassium levels were observed after treatment with metanil yellow for 8 weeks. The study also showed a significant decrease in total protein and albumin levels in rats exposed to both sodium nitrite and metanil yellow. Furthermore, increased in liver enzyme, AST, ALT and ALP activities in sodium nitrite and metanil yellow treated groups. In addition, decrease in catalase and glutathione reduced, while increase in lipid peroxide and nitric oxide activity were showed. Histological changes of the sodium nitrite treated group revealed no pathological lesions of kidney cells and metanil yellow treated group are within normal limits with mild glomerular congestion, whereas the liver tissues of sodium nitrite and metanil yellow treated groups showed mild fatty change, central vein congestion, and sinusoidal congestion. On the other hand, the daily intake of natural bees honey at a dose of 2.5g/ Kg body weight resulted in sufficient amelioration against the adverse effects of food additives as indicated by the observed improvement in all tested biochemical parameters and increase in antioxidant enzyme (catalase) and glutathione reduced and decrease in oxidant enzyme (lipid peroxide) and nitric oxide. In conclusion, this study revealed that the use of natural bees honey has the ability of preventing kidney dysfunction induced by food additives. |
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Supervisor |
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Prof. Dr. Jalaluddin A Jalal Awlia Khan |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1434 AH
2013 AD |
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Added Date |
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Wednesday, November 6, 2013 |
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Researchers
| نجاح سعد العتيبي | Alotaibi, Najah Saad | Researcher | Master | |
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