|
Document Type |
: |
Thesis |
|
Document Title |
: |
Multiplex PCR for Diagnosis of Human Brucellosis متعدد تفاعل سلسلة البوليميراز (بي سي ار) لتشخيص البروسيلا الإنسان |
|
Subject |
: |
Biochemistry department |
|
Document Language |
: |
Arabic |
|
Abstract |
: |
Introduction: Brucellosis is a zoonotic disease transmittable to humans globally . Brucella, Gram negative cocco bacilli, infested humans through the consumption of unpasteurized milk, cheese, and meat. Diagnostically, there are four mutual pathogenic Brucella species for both human and animals: Brucella melitensis, Brucella abortus, Brucella suis, and Brucella canis. Multiplex PCR is powerful differentiative technique to monitor the presence of Brucellea. In this study, two sequences of local Brucellea are targeted for their pathogenic contributions brucellosis is endemic, with more than 8000 cases reported each year to public health authorities.
Methods: Total of 101 Acute Fibril Illness (AFI) serum samples were collected from two different cities in the Tabuk and Jeddah west regions of the Kingdom of Saudi Arabia. All samples were subjected to DNA extraction, and Brucella genus specific B4/B5 DNA amplification. Positive B4/B5 patients subjected to multiplex species-specific Brucella amplification.
Results: concerning north west's samples, (81.9%) of AFI samples were confirmed to be positive. Positive B4/B5 patients subjected to multiplex species-specific Brucella amplification, which detects that B. abortus amplification in (10%) and B. melitensis in (8%) of the samples, while (82%) of samples showed double product of both B. abortus and B. melitensis. while all samples subjected form Jeddah proved to be non Brucella.
Conclusions:. This study recommends two stages PCR diagnosis for human brucellosis. Moreover; epidemiological database must be established in the Kingdom of Saudi Arabia to investigate such AFI patients for further information. |
|
Supervisor |
: |
Prof. Khalid O. Abulnaja |
|
Thesis Type |
: |
Master Thesis |
|
Publishing Year |
: |
1434 AH
2013 AD |
|
Added Date |
: |
Thursday, November 7, 2013 |
|
Researchers
| باسم يحيى القشقري | AL-Gashgari, Basim Yahya | Researcher | Master | |
|