Document Details

Document Type : Thesis 
Document Title :
Seroepidemiological Study of Dengue Fever among Students Attending Medical Administration of King Abdulaziz University
دراسة وبائية مصلية لحمى الضنك بين الطلاب المراجعين للادارة الطبية بجامعة الملك عبدالعزيز
 
Subject : biological sciences department 
Document Language : Arabic 
Abstract : Dengue fever virus is considered the most important arbovirus in terms of morbidity, mortality. Dengue is transmitted by female Aedes aegypti mosquito and occurs in epidemic and endemic proportions throughout tropical and subtropical regions of the world. There are four distinct types but antigenically related serotypes of dengue viruses, (DEN-1, DEN-2, DEN-3, and DEN-4). In 1994, dengue virus was isolated in Jeddah, Saudi Arabia, for the first time. To this point a need for seroepidemiological study were raised specially of the recent continuous epidemic in Jeddah province started in 2006 until now to determine the prevalence and incidence of dengue infection among several populations. In addition a serotyping test like immunofluorescence (IFA) and immunoassay (ELISA) tests become demandable due to the lack of commercial supply. The collected blood samples from participant were tested by Indirect IgG-ELISA method to measure the dengue IgG antibodies. Home ELISA and in-house IFA performed on positive dengue IgG-Abs samples to detect the serotypes of dengue. Forty samples out of 342 were positive for dengue IgG-Abs. All positive samples were living either in Jeddah (87.5%), Makkah (12.5%). Males constituted 60% of the positive subjects while females constituted the remaining 40%. The most prevalent serotype by this assay was type-1 (60.6%) followed by type-2 (10%) and type-4 (7.5%) the remaining samples were mixed infection between types-1 and 2 (12.5%) and types 1, 2,3 and 4 were (10%). This study showed the high prevalence (40/342) 11.7% of past dengue infection in apparently healthy individuals who did not report having dengue infection or dengue like symptoms before. The male gender and the age above 30 years old factors found to have significant correlation with the prevalence of dengue. The study also showed that ELISA serotyping assay is more specific and more sensitive than IFA in the detection of dengue serotypes. 
Supervisor : Dr. Nezar Abdulmoty Redhwan 
Thesis Type : Master Thesis 
Publishing Year : 1436 AH
2014 AD 
Co-Supervisor : Dr. Esam Ibraheem Azhar 
Added Date : Wednesday, December 24, 2014 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
رائد عبد الوهاب بديرةBadierah, Raied AbdulwahabResearcherMaster 

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