Document Details

Document Type : Thesis 
Document Title :
MOLECULAR CHARACTERIZATION OF ANTIMICROBIAL RESISTANCE OF ENTEROCOCCUS SPP. ISOLATED FROM THE LOCAL COMMUNITY AND HOSPITAL PATIENTS
التوصيف الجزيئي لمقومة المضادات في أنواع إنتيروكوكاس المعزولة من المجتمع المحلي ومرضى المستشفيات
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : Molecular characterization of antibiotics resistance genes of E. faecalis and E. faecium isolated from King Abdulaziz University hospital patients MUHAMMAD FARMAN JANNAT MIR Supervisors: Dr. Rashad Rizq Al-Hindi Dr. Esam I. Azhar Antimicrobial resistance is a serious health problem that is rapidly escalating in all kinds of microorganisms among community and hospitalized patients across the world including Saudi Arabia. It potentially confines the effectiveness of many widely used antibacterial drugs. The most widespread multidrug-resistant bacteria are the Gram- positive methicillin resistant Staphylococcus aureus (MRSA). However, antimicrobial resistance among other groups of bacteria, including Enterococcus spp. is growing and pose significant treatment problems in the health care system of Saudi Arabia. Study of molecular surveillance for antimicrobial resistance is necessary to detect emerging resistances and to support management of infections in hospitals. In this study, a molecular epidemiology program was established to monitor the trends of antibiotic resistance in Enterococcus spp. The target strains were cultured from clinical specimens obtained from large tertiary-care hospital of King Abdulaziz University hospital (KAUH). Isolates were identified by high-throughput MALDI-TOF using a VITEK- MS (bioMérieux, France) system and genotyping of the enterococcal isolates was performed using PFGE in a CHEFDR II apparatus (Bio-Rad, USA). Genomic DNA was extracted from E. faecalis isolates using 130 UltraClean® Microbial DNA isolation kit (MO BIO Laboratories, Inc. USA). Genomic libraries were prepared using Nextera XT DNA library preparation kit (Illumina, Inc. USA) and sequencing was performed using V3, 2×300 bp chemistry on a MiSeq platform (Illumina, Inc., USA). Genes responsible for AMR to vancomycin, aminoglycoside, and quinolones were amplified from the phenotypically resistant enterococcal isolates using primers. ARGs were identified using ResF 
Supervisor : Dr. Rashad Al Hindi 
Thesis Type : Doctorate Thesis 
Publishing Year : 1440 AH
2019 AD 
Added Date : Monday, February 25, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
محمد فرمانFarman, Mohammed ResearcherDoctorate 

Files

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 43977.pdf pdf 

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