Document Details

Document Type : Thesis 
Document Title :
MOLECULAR CHARACTERIZATION OF BARLEY CULTIVARS USING ORGANELLE SNPS”
"التوصيف الجزيئي لأصناف نبات الشعير باستخدام تعدد أشكال النيوكليوتيدات المفردة الخاصة بالعضيات"
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : The present study aimed at detecting single nucleotide polymorphisms (SNPs) in the mitochondrial and plastid genome sequences generated via next-generation sequencing for the characterization of selected barley genotypes and test the theory of heteroplasmy. The analysis involved seven cultivated barley (Hordium vulgare subsp. vulgare) (VG) and one wild (H. vulgare subsp. spontaneum) (SP) genotypes. The mitochondrial and plastid genomes of the eight barley genotypes were sequenced using Illumina HiSeq 2000 platform of which around 480 million paired-end reads of 100 nt each were generated across genotypes. Sequences of each genotype were separately aligned to the published barley plastid and mitochondrial reference genomes, and SNPs were detected. The results indicated the efficiency of using SNPs as a molecular marker in distinguishing among barley genotypes. Specific mitochondrial SNPs were determined in six out of the eight genotypes, while only four for plastid SNPs. Giza131 was the only genotype with no specific mitochondrial or plastid SNPs, while Giza130 showed the largest number of specific mitochondrial (7) and plastid (5) SNPs. Phylogenetic trees generated from SNPs of the two genomes indicated the close relationship between Giza129 and Giza 130, however, the mitochondrial SNPs indicated a better discrimination among genotypes. Interestingly, SP was not clearly discriminated among genotypes. The study validated the heteroplasmy theory in mitochondrial and plastid genomes. 
Supervisor : Prof. Ahmed Bahieldin 
Thesis Type : Master Thesis 
Publishing Year : 1440 AH
2019 AD 
Co-Supervisor : Dr. Hani Mohammed Ali 
Added Date : Tuesday, April 23, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
بشائر عصام العطاسAl-attas, Bashaer EsameResearcherMaster 

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