Document Details

Document Type : Thesis 
Document Title :
MOLECULAR CHARACTERIZATION OF CATHARANTHUS ROSEUS VARIETIES USING CHLOROPLAST SNPS”
التوصيف الجزيئي لأصناف نبات الوينكا باستخدام تعدد أشكال النيوكليوتيدات المفردة الخاصة بالبلاستيدات
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : Catharanthus roseus is a widespread ornamental plant due to its glamorous flowers colors. More importantly, it is a medicinal plant that produces pharmaceutical compounds including the powerful species-specific antitumor drugs, vinblastine and vincristine. Varieties of C. roseus show differences in their physiological and biochemical characteristics including the production of dissimilar amounts of these antitumor drugs. Characterization and identification of varieties depend on flowers morphological characteristics such as petals and flowers eyes colors. However, most of this medicinal plant sales are carried out at the vegetative stage, causing a major obstacle for consumers since it is impossible for varieties to be morphologically characterized at this stage. Therefore, this study was designed to develop alternative faster and more reliable method for characterization. High-throughput next generation sequencing was utilized to detect chloroplast SNPs that are unique to each variety for molecular characterization. Total DNA of eight C. roseus varieties was sequenced by using Illumina HiSeq 2000 platform. Resulted sequences for each variety were aligned separately to the chloroplast reference genome (KC561139), followed by examining SNPs variants. The results identified unique SNPs for all eight varieties. In addition, intra-varietal SNPs were detected confirming the applicability of the heteroplasmy theory in chloroplast genome of this species. This investigation might provide valuable insights into the utilization of the resulted molecular markers in germplasm identification, breeding programs and cultivar-right protection. Finally, further studies could also be conducted using mitochondrial or nuclear SNPs for molecular characterization of C. roseus varieties. 
Supervisor : Dr. Ahmed Mohammed Emara 
Thesis Type : Master Thesis 
Publishing Year : 1440 AH
2019 AD 
Co-Supervisor : Dr. Thana Khalid Khan 
Added Date : Tuesday, April 23, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
أريج عادل صعيديSaeedi, Areej AdelResearcherMaster 

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