Document Details

Document Type : Thesis 
Document Title :
Molecular Characterization and Antimicrobial Resistance of Extended Spectrum-β-Lactamases Producing Escherichia coli Strains in Saudi Arabia
التوصيف الجزيئي والمقاومة للمضادات في سلالات بكتيريا Escherichia coli المُنتجة لإنزيمات β-lactamases واسعة الطيف في المملكة العربية السعودية
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : Antimicrobial resistance is a serious health problem that is rapidly escalating across the world including Saudi Arabia. The extended spectrum-β-lactamases (ESBLs) are the enzymes that make bacteria resistant to currently available advanced generations of cephalosporin antibiotics. The aim of this study, a molecular characterization to monitor the trends of antibiotics resistance and ESBL producing Escherichia coli (E. coli) genotyping. In total, 213 clinical isolates of ESBL-positive E. coli recovered from clinical specimens that were collected from King Abdulaziz University Hospital during 2015G. The isolated strains were screened for antibiotics susceptibility to provides the multidrug resistant profile of ESBL positive E. coli isolates. The resistant strains genotyping was performed by using multi locus sequence typing to identify the common ESBL positive E. coli clones circulating in our health care facilities. The information evaluated in a global context by comparing finding of this study with international data. The results of antibiotics susceptibility indicated high resistance to cefalotin and ampicillin with percentage (≥ 99%), followed by ceftriaxone (97%). While there is no resistance to imipenem and meropenem (0%) among isolates. Varying levels of resistance was observed against the other tested antibiotics, such as the resistance to ceftazidime and ciprofloxacin was around (70%). ST131 was identified as dominant clonal type (35%) among ESBL producing E. coli isolates. All the ST131 associated isolates were resistant to ciprofloxacin and were sensitive to imipenem and tigecycline. In conclusion, all tested ESBL-positive E. coli isolates were found to be multidrug resistant. In consistent to worldwide studies, ST131 is identified as dominant STs and responsible of acquired infections in both hospitals and community. However, the antibiotics from carbapenems group could be used as treatment option in the multidrug resistance ESBL- E. coli in the emergency. This study is considered one of the epidemiological studies to provide information to determine the pattern of resistance in the most infectious bacteria acquired in hospitals and the community. Further, it provides basic knowledge about the genetic profiling of multiple locally dispersed isolates that will support the control to any infection in the future. 
Supervisor : Dr. Saad Berki Al-Masaudi 
Thesis Type : Master Thesis 
Publishing Year : 1440 AH
2019 AD 
Co-Supervisor : Dr. Mohammed Yasir Noorwali 
Added Date : Sunday, May 5, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
عبير محمد عجلانAjlan, Abeer MohammedResearcherMaster 

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