Document Details
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Document Type |
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Thesis |
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Document Title |
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Molecular identification and characterization of different Dengue virus serotypes infecting human in Jazan Area Kingdom of Saudi Arabia التحديد والتوصيف الجزيئي في مختلف أنماط فيروس حمى الضنك الذي يصيب الإنسان في منطقة جازان، المملكة العربية السعودية |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Dengue virus (DENV) infection is an endemic mosquito borne viral disease, regularly occurs in tropical regions of the world. DENV was endemic in many countries included Kingdom of Saudi Arabia (KSA). In KSA, the disease outbreaks have been reported in different time during 1994-2016 with significant impact which associated with epidemics, great economic and social impacts. At least three DENV serotypes have been reported in the KSA with high morbidity and high mortality rates. However, molecular detection and characterization of dengue virus among human in Jazan Area, KSA, have not been extensively studied. The development of simple, affordable, highly sensitive and specific and applicable diagnostic test is needed. RT-PCR based detection at field level, having significant impact on the future control strategies has been tested in the current study. The present study was conducted 189 blood samples collected from febrile patients of different age, sex and nationality during their admission into hospitals (13 hospitals at Jazan area). Sera from the all blood samples collected were subjected to ELISA assay, using three different commercial kits. RNA was extracted from blood samples using High Pure Viral Nucleic Acid Kit, subjected to one step RT- PCR molecular analysis using dengue consensus serotype-specific primers. Dengue virus RNA from RT-PCR positive samples was extracted, purified and their nucleotide sequences were aligned with NCBI data base. The linear results of IgG IELISA showed increasing pattern by age from young to elder age groups and the results of Nonstructural protein 1 (NS1) and Immunoglobulin M (IgM) on the other hand showed different pattern, which were increased from young to young adult, decreased to worth the adult and not detected by in the elder age group. In different serologic test NS1, IgM and IgG respectively, high positive cases were detected in male 25 (21.4%), 35 (30.5%), 57 (48.3%) compare to female 1 (15.3), 20 (28.2%), 22 (31.0%). High dengue incidence was detected in 7 months and low in 5 months (August, September. November, December and January). The prevalence of the dengue primary course infection during different months was significantly correlated to the rain fall. DENV1 was more commonly detected (79.7%) followed by DENV2 (16.5%) and concurrent mixed infection with both DENV1 and DENV2 (3.8%) but DENV3 and DENV4 were not detected in all collected blood samples. RT-PCR analysis has resulted 79 (64.2%) DENV positive samples and 44 (35.8%) negative out of 123 tested samples. DENV1 and DENV2 were detected by RT-PCR and were 97% and 99% respectively based on sequence similarity in Gen-Bank and their phylogenetic similarity, phylogenic trees were generated. RT-PCR was specific (96.8%) and the sensitive (95.1%) compared to NS1. We concluded, age is a crucial variable factor in secondary DENV infectivity and in case fatality. Concurrent mixed infection with multiple DENV serotypes (DENV1, DENV2) were detected for the first time in Jazan area. |
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Supervisor |
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Prof. Mohammed Ishtiaq Qadri |
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Thesis Type |
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Doctorate Thesis |
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Publishing Year |
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1440 AH
2019 AD |
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Co-Supervisor |
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Prof. Khalid Al-Ghamdi |
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Added Date |
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Sunday, May 26, 2019 |
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Researchers
| عبد العزيز محمد حكمي | Hakami, Abdulaziz Mohammed | Researcher | Doctorate | |
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