Document Details

Document Type : Thesis 
Document Title :
Molecular Mechanism of Dimethyl Sulfoxide (DMSO) Potentiating Effect on Cytotoxicity of Cisplatin Against the Growth of Lung Cancer Cells.
الأليه الجزيئيه لنشاط الداي ميثيل سلفوكسيد المحفز على التأثير السام للسيسبلاتين على نمو خلايا سرطان الرئة
 
Subject : Faculty of Medicine 
Document Language : Arabic 
Abstract : Cisplatin is a potent anticancer agent with high therapeutic efficacy against many kinds of tumours. Its use is limited by its nephrotoxicity and ototoxicity. The present study was designed to assess the role of sulfur containing agent dimethyl sulfoxide (DMSO) on sensitization of Lung carcinoma (A549) to the action of Cisplatin. The aim of this study was directed to explore whether the DMSO could enhance the cytotoxic effect of Cisplatin against the growth of LC cell line (A549) and to investigate the possible mechanisms of interaction between DMSO and Cisplatin regarding Cisplatin cytotoxicity, apoptosis induction, P-glycoprotein activity, Cisplatin cellular uptake and cell cycle phase disturbance in the presence or absence of DMSO. DMSO increased the cytotoxic activity of cisplatin against the proliferation of lung cancer cells with IC50 16.07 as compared to 23.4 when treated with cisplatin alone. Combination treatment of Cisplatin either 5 or 20 µg/ml and DMSO (10%) inhibited p-glycoprotein as measured by increase in Rho-123 uptake by 1.6 and 1.3 fold, respectively compared to cells treated with Cisplatin alone. In addition, treatment of A549 cells with DMSO (10%) and Cisplatin 5 or 20µg/ml showed a significant increase in apoptosis to 81% and 84 % respectively, compared to 74% and 77% when cells treated with Cisplatin alone. Moreover, combination treatment of Cisplatin 5 or 20 µg/ml and DMSO (10%) showed a significant increase in percentage of cells in G0/G1 phase estimate to 61.9% and 70.3% respectively, compared to 47.5 % and 64.5% when cells treated with Cisplatin alone. In conclusion, DMSO treatment enhanced the cytotoxic activity of cisplatin against the growth of A549 by inhibition of p-glycoprotein activity and disturbance of cell cycle . 
Supervisor : Dr. Sameer E. Al-Harthy 
Thesis Type : Master Thesis 
Publishing Year : 1440 AH
2019 AD 
Co-Supervisor : Prof. Abdel-Moneim M. Osman 
Added Date : Wednesday, July 24, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
ريم مصطفى محمدMohammed, Reem MustafaResearcherMaster 

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