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Document Type |
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Thesis |
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Document Title |
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IN VITRO STUDY THE ANTI-ATHEROGENIC ACTIONS OF THYMOQUINONE IN THP-1 MACROPHAGES دراسة معملية لتأثير الثيموكينون كمضاد لتصلب الشرايين في الخلايا الضامة |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Atherosclerosis, an inflammatory disease of the arteries is responsible for 37% of all deaths in Saudi Arabia. Thymoquinone (TQ), derived from Nigella Sativa is claimed to have anti-inflammatory and cardio-protective effects. In this study, we used THP-1 cell line (a well-established model to study atherosclerosis) and evaluated the anti-inflammatory effects of TQ. THP-1 cells (4x104 cells/well/96-well plate) were exposed to TQ at different concentrations (1, 2.5. 5, 7.5 and 10μM) for 24h and cell inhibition assay were performed using WST-1 and LDH assays. Cell migration was done using Boyden chamber where THP-1 cells (1x106 cells/well) were plated in the upper chamber and were exposed to medium with either chemo-attractant (MCP-1) or inhibitors (TQ5μM and TQ10μM) in the lower chamber, for 3h. Real-time PCR was done to study the expression of ICAM-1 and MCP-1 following treatment of THP-1 cells (3x106 cells/25cm2) with TQ (2.5. 5, and 10μM) for 24h. WST-1 assay demonstrated concentration dependent mean decreases in THP-1 cell line by (28.69%, 57.25%, 69.09%, 70.61% and 71.83%) for TQ (1, 2.5. 5, 7.5 and 10μM) respectively. LDH assay demonstrated concentration dependent mean decreases in THP-1 cell line by (4.42%, 17.37%, 30.37% and 31%) for TQ (2.5. 5, 7.5 and 10μM) respectively. Cell migration assay demonstrated that MCP-1 led to migration of THP-1 cells while TQ inhibited them. qRT-PCR demonstrated decreased expression of ICAM-1 and MCP-1 with higher concentrations of TQ compared to untreated control. TQ also decreased cell migration and expression of genes that are involved in the pathogenesis of atherosclerosis. TQ, therefore has anti-inflammatory effects and can be considered for use against atherosclerosis. |
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Supervisor |
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Dr. Etimad Huwait |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1441 AH
2019 AD |
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Added Date |
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Thursday, September 19, 2019 |
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Researchers
| نوف أحمد غروي | Gharawi, Nouf Ahmed | Researcher | Master | |
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