Document Details

Document Type : Thesis 
Document Title :
IN VITRO STUDY OF THE BIOLOGICAL ACTIVITY OF MARINE ALGAE FROM RED SEA
دراسة معملية للنشاط البيولوجي لطحالب بحرية من البحر الأحمر
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : The drug discovery using natural products which in the form of medicinal plants or marine organism still an important target for recent research. This study investigated anticancer activity of algae extracts obtained from Red sea at Jeddah. Aqueous and methanol extracts of Dictyota ciliolate (DC) were tested on HCT-116 and HepG2 cell lines using WST-1 assay. Aqueous and methanol extracts at doses of 0.05, 0.1, 0.5 and 1mg/ml and positive control of 0.3% H2O2 at doses of 0.5mg/ml for 24, 48 and 72h (for the two cell lines). Aqueous extract showed no effect against HCT116 or HepG2. While methanol extract showed an anticancer effect on HCT116 and HepG2 at doses of 0.5mg/ml comparable to aqueous extract at 24, 48 and 72 hours. DNA fragmentation analysis of algae extract treated cells showed a laddering pattern, which is characteristic of apoptosis, indicating internucleosomal DNA degradation. The antitumor effect of methanol extracts is promising provided information about the molecules responsible for this activity and their mechanisms of action, paving thus the way to develop new herbal medicines and forming the foundation for future studies. It is recommended to investigate the gene expression and antioxidant effect in response to this extract as promising as antitumor agents from natural sources. 
Supervisor : Prof. Said Salama Moselhy 
Thesis Type : Master Thesis 
Publishing Year : 1441 AH
2019 AD 
Co-Supervisor : Dr. Etimad Abbas Huwait 
Added Date : Wednesday, October 2, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
مروج رأفت مداحMaddah, Morog RafatResearcherMaster 

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