Document Details

Document Type : Thesis 
Document Title :
ISOLATION AND CHARACTERIZATION OF INDIGENOUS BACILLUS THURINGIENSIS STRAINS FOR BIOCONTROL OF AEDES AEGYPTI
عزل وتوصيف سلالات محلية من Bacillus thuringiensis للمكافحة الحيوية للبعوض المصري
 
Subject : faculty of science 
Document Language : Arabic 
Abstract : Bacillus thuringiensis (Bt) is a rod-shaped, Gram positive bacterium that produces insecticidal crystal proteins also known as δ-endotoxin during sporulation. Crystal proteins or Cry proteins are toxic to a wide range of insect pests such as lepidopteran insects, beetles and mosquitoes etc. Due to their high specificity, efficacy and safety, these Cry proteins have been successfully used as a biopesticide to control various insect pests for the last six decades. Significance of the Cry proteins has motivated the search for new strains of Bacillus thuringiensis from diverse habitats around the globe to identify new crystal proteins with unique target species. Our goal of the proposed project was to isolate and characterize the new endogenous Bt strains producing crystal protein with more potent mosquitocidal activity as compared to the currently available Bt strains, from different regions of Saudi Arabia. Soil samples were collected from various regions of Saudi Arabia and isolation of Bt strains was carried out using the selective sodium acetate medium and heat treatment method. Around 85 soil samples were processed and screened which resulted in the isolation of 3 Bt isolates. Biochemical tests and phylogenetic analysis based on 16S DNA gene sequencing revealed that the three bacterial isolates were Bacillus thuringiensis strains. A crystal-spore differential staining revealed the expression of crystal proteins in all the three new endogenous Bt isolates during the sporulation phase. Spore-crystal protein mixture of all the three Bt isolate was shown to possess insecticidal activity against the larvae of Aedes aegypti. Moreover, tests under light microscopy showed that crystal proteins are present in all three isolates and have a fatal effect on the Aedes aegypti mosquito larvae. 
Supervisor : Dr. Mohammad H. Fakieh 
Thesis Type : Master Thesis 
Publishing Year : 1441 AH
2019 AD 
Co-Supervisor : Dr. Naser A. Alkenani 
Added Date : Thursday, December 12, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
جهاد أحمد دينDin, Jihad AhmedResearcherMaster 

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