Document Details

Document Type : Thesis 
Document Title :
ISOLATION AND IDENTIFICATION OF NOVEL BACTERIAL CONSORTIA RESPONSIBLE FOR DEGRADING PYRENE
عزل وتوصيف سلالات بكتيرية جديدة لها القدرة على تحليل البيرين
 
Subject : faculty of science 
Document Language : Arabic 
Abstract : Pyrene is a polyromantic hydrocarbon ubiquitously found in soils. Bioremediation of this lethal and toxic compound is of utmost importance due to its toxicity toward humans. It is imperative to identify and isolate bacterial species that readily degrade pyrene present in the soil. In this study soil samples was collected from different sites in the Madinah region. These studied soil samples were used to the presence of bacterial species that readily degrade pyrene. Isolates were thoroughly studied for the degradation of Pyrene using Spectrophotometer, 2,6-Dichlorophenolindophenol Assay (DCPIP), and dry weight method. About 18 bacterial isolates were isolated from soil samples of Madinah. Among them, only P12 and P7 show high degradation activity. Furthermore, the potent strains were identified using biochemical and 16s rRNA as Pseudomonas stutzeri strain YA 8805 (MK648317) and Pseudomonas sp strain YA 2364 (MN055898) respectively. Results show that both of these strains change color from blue to white in the presence of 2,6-Dichlorophenolindophenol dye (DCPIP) compare to the control one. The optical density of these strains was also high at 600 nm wavelength. HPLC as quantitative analysis shows that one of the selected isolate P12 shows 100 % degradative activity compare to the control one. The dry weight confirms this strain can degrade pyrene. Our study concludes that these strains can be used to clean the environment. 
Supervisor : Dr. Hani Mohammed Ali 
Thesis Type : Master Thesis 
Publishing Year : 1441 AH
2019 AD 
Co-Supervisor : Dr. Yasir Anwar Ul haq 
Added Date : Sunday, December 22, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
عبد الله عبيد الحربيAl harbi, Abdullah ObaidResearcherMaster 

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