Document Details

Document Type : Thesis 
Document Title :
PROPHYLACTIC EFFECT OF DATE PALM FRUIT EXTRACT AGAINST DOXORUBICIN-INDUCED HEPATOTOXICITY IN MALE WASTER ALBINO RATS
التأثير الوقائي لمستخلص ثمار التمر إزاء التسمم الكبدي المستحث بالدوكسوروبيسين في ذكور الجرذان البيضاء
 
Subject : faculty of science 
Document Language : Arabic 
Abstract : Background: This study evaluated the possible prophylactic efficacy of date palm fruit aqueous extract (DPFAE) in alleviation of the hepatotoxicity induced by doxorubicin (DOX) injection. Materials and methods: Wistar albino rats were equally and randomly divided to six groups: group 1: the untreated control, groups 2&3: rats were received daily oral prophylactic doses (0.75 & 1.5 of mg/kg bw of DPFAE) respectively for 30 days. Rats of groups 4, 5 & 6 were injected with a single intra-peritoneal dosage of DOX (15 mg/kg) on the 30th day of the study. However, rats of groups 5 & 6 received a daily oral protective doses of DPFAE (0.75 & 1.5 mg/kg bw respectively) for 30 days. Serum samples were used for estimation of ALT, AST, γ-GT and ALP by the optimized standard kinetic method. T. bilirubin, Total protein, Albumin were estimated by direct colorimetric method. Liver was divided into three fragments. One fragment was soaked immediately into 10% buffered neutral formaldehyde solution and managed for histopathologic examination, the second fragment was utilized for DNA extraction and the third fragment was utilized for preparation of liver tissue homogenate. Results: Treatment with DPFAE significantly ameliorated the altered liver enzymes and significantly improved rest of the liver profile as well as oxidative stress markers. This was together with major improvement in the hepatic histopathological features. Conclusion: The present results revealed that DPFAE seems to improve DNA damage of DOX-induced hepatotoxicity indicating the influence of DPFAE in restoring the functional ability of hepatic cells towards normalization. DPFAE was able to deactivate reactive oxygen species, suppressing lipid peroxidation and DNA injury. 
Supervisor : Dr. Mohamed Kamel Abo-Golayel 
Thesis Type : Master Thesis 
Publishing Year : 1441 AH
2020 AD 
Co-Supervisor : Dr. Othman Abobaker Baothman 
Added Date : Tuesday, February 11, 2020 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
أحمد محمد الفتنيAl Fattani, Ahmed MohammedResearcherMaster 

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