Document Details

Document Type : Thesis 
Document Title :
MOLECULAR IDENTIFICATION OF MICROBIOTA IN NORMAL AND PATIENT S HUMAN ORAL CAVITY
التعريف الجزيئي للميكروبات في تجويف فم الانسان العادي والمريض
 
Subject : faculty of science 
Document Language : Arabic 
Abstract : The oral cavity of human can be exposed to a different kind of environmental microorganisms, which is inhaled by air, drink water and ingested food. To understand how oral microbiota, maintain or dispose of health, one major tool is to analyze the microbial population. The present study aims to detect and identify microbes in human saliva microbiota using phenotypes, gram stain and molecular techniques using 16S rRNA gene sequencing. A total number of 100 samples were collected from medical school male students and employees volunteers at Jeddah, Saudi Arabia in March 2017. The bacterial sources correlate species with health conditions, smokers (S), influenza-infected (I) and normal group (N). A broad diversity of bacteria was characterized, including representatives of two different bacterial phyla; Firmicutes such as Staphylococcus, Proteobacteria such as Klebsiella species. High dominance among the identified genera of Staphylococci 30%, followed by Klebsiella 15%, while unculturable species showed 50% dominance. There was 100% correlation of the genera Klebsiella with smokers group. Staphylococci genera which were dominant in the normal and influenza-infected group disappeared completely in the smokers group. Comparing the morphology data observed with molecular 16S rRNA identities, the results agreed with the genera identified. Our study suggests that smoking alters bacterial acquisition and oral mucosal colonization in favor of periodontal pathogens. Awareness should be created for the public on the health implication of smoking and poor oral practice. 
Supervisor : Prof. Mohamed Morsi M. Ahmed 
Thesis Type : Doctorate Thesis 
Publishing Year : 1441 AH
2020 AD 
Co-Supervisor : Prof. Mohammed Hamed Zainy 
Added Date : Wednesday, March 11, 2020 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
محمد إبراهيم العسيريAlassiri, Mohammed IbrahimResearcherDoctorate 

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