Document Details

Document Type : Thesis 
Document Title :
MOLECULAR MECHANISMS UNDERLIE THE ANTICANCER ACTIVITIES EXERTED BY THYMOQUINONE AGAINST ACUTE MYELOID LEUKEMIA CELLS: ROLE OF WT1 AND BCL2 GENES
الآليات الجزيئية المسؤولة الكامنة وراء الانشطة المضادة للسرطان المحدثة بالثايموكينون ضد خلايا سرطان الدم النخاعي الحاد: دور جيني Bcl2 وWT1
 
Subject : faculty of science 
Document Language : Arabic 
Abstract : Acute myeloid leukemia (AML) is an aggressive and heterogeneous disease characterized by an abnormal proliferation and impaired differentiation of the myeloid precursor cells. The outcome for most AML patients remains poor with high relapse rates and chemotherapy remains the first line treatment for AML. The Wilms tumor WT1 and the anti-apoptotic BCL2 genes are upregulated in AML and are known be involved in apoptosis inhibition. In the present study we evaluated the molecular mechanisms underlie the anti-proliferative and pro-apoptotic activities exerted by thymoquinone (TQ), the major biologically active compound of the black seed oil on acute myeloid leukemia cell line-HL60. Cell proliferation was determined by WST-1 assay and apoptosis rate was assessed by flow cytometry using annexin-V/7AAD staining. The expression of target genes was analyzed by real-time RT–PCR analysis. TQ significantly reduced HL60 cell viability and induced apoptosis in a dose and time-dependent manner. In order to decipher the molecular mechanisms underlie the anti-cancer activities induced by TQ in AML cells, we investigated its effect on the expression of WT1 and BCl2 genes. TQ significantly decreased the expression of WT1 and BCL2 genes in a dose and time-dependent manner. In summary, these findings suggest that TQ induces cell proliferation inhibition and apoptosis in acute myeloid leukemia cells most likely through targeting the apoptosis-related WT1 and BCl2 genes and also suggest that TQ could be a promising strategy for AML therapy. 
Supervisor : Dr. Mahmoud Alhosin 
Thesis Type : Master Thesis 
Publishing Year : 1441 AH
2020 AD 
Co-Supervisor : Dr. Mustafa Adnan Zeyadi 
Added Date : Thursday, March 12, 2020 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
مشاري غازي مصليMusall, Mishary GhaziResearcherMaster 

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