Document Details

Document Type : Thesis 
Document Title :
Isolation and identification of pathogenic bacteria showing resistance against disinfectants
عزل وتعريف البكتريا الممرضة التي تظهر مقاومة للمطهرات
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : Disinfectants used extensively across the world. Overuse and misuse of disinfectants could lead to disinfection failure, problems to public health and may cause food-borne disease outbreaks by pathogenic bacteria. This study investigates the presence of pathogenic bacteria in food facilities and their ability to show resistance against disinfectant products. Bacteria were isolated from five food facilities and identified using 16s rRNA. Minimum inhibitory concentration and minimum bactericidal concentration was determined using macro-dilution broth following the Clinical and Laboratory Standards Institute guidelines and six disinfectants used for this experiment were sodium hypochlorite, chloroxylenol and benzalkonium chloride were available on two formulations (commercially and pure). Isolates were exposed to sub-inhibitory concentration to observe the effect of sub-inhibitory concentration on increasing bacterial resistance to disinfectants. Out of selected strains, three were identified as pathogenic bacteria Klebsiella pneumoniae C10, Staphylococcus sciuri YY64 and Macrococcus caseolyticus CCM7927. Minimum inhibitory concentrations results showed that Klebsiella pneumoniae have very high tolerance to both commercially benzalkonium chloride and chloroxylenol, for minimum bactericidal concentrations Klebsiella pneumoniae showed high resistance to sodium hypochlorite, benzalkonium chloride, and commercially obtained chloroxylenol while Staphylococcus sciuri showed high resistance to pure obtained chloroxylenol. Exposing to sub-inhibitory concentration increased the MIC and MBC on 66% of the result. The highest increase to MIC was S.sciuri as their MIC value to benzalkonium chloride increased sixteen-fold (from 1.07 µg/ml to 17.3 µg/ml), and for MBC result K.pneumoniae showed eight-fold increase to chloroxylenol (from 75 µg/ml to 600 µg/ml). Based on these results K.pneumoniae were the most resistance bacteria to disinfectants. These results suggest that the misuse of disinfectant products for disinfection processes may increase bacterial resistance to disinfectant which could lead to disinfection failure and cause threat to public health. 
Supervisor : Dr. Bassam al Johny 
Thesis Type : Master Thesis 
Publishing Year : 1441 AH
2020 AD 
Added Date : Monday, May 25, 2020 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
عبدالإله مصلح الخزاعيal Khuzaee, Abdulelah ResearcherMaster 

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