Document Details
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Document Type |
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Thesis |
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Document Title |
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Possible protective effect of Matricaria chamomilla L. against alteration of thyroid gland in rat model of polycystic ovary syndrome التأثير الوقائي المحتمل للبابونج (ماتريكاريا كاموميلا) ضد تغيرات الغدة الدرقية في نموذج تكيس المبيض في الجرذان |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Polycystic ovary syndrome (PCOS) is a challenging clinical problem among women that might be associated with thyroid gland disorders. Matricaria chamomilla L. was used as a traditional medication for PCOS based on its high contents of antioxidants.
PCOS was induced by intramuscular injection (I.M) of estradiol valerate (EV) in a dose of (0.2 mg) in (0.2 ml) corn oil. Twenty-four adult female Wistar rats (200-220g) were divided into four groups. G1; (control) received 0.2 ml of corn oil, G2; positive control (PCOS), G3; PCOS+ M. chamomilla (75mg/kg), G4; PCOS + metformin (140mg/kg). Treatments were given daily for 4 weeks orally. Serum follicle stimulating hormone (FSH), luteinizing hormone (LH), testosterone, thyroid stimulating hormone (TSH), thyroxine (T4), triiodothyronine (T3), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione (GSH), and malondialdehyde (MDA) were determined. Light and electron microscopic features of thyroid follicular cells besides immunohistochemical staining for caspse-3 and proliferating cellular nuclear antigen (PCNA) expression were matched with hormonal analysis. Data were expressed as mean ± SE and were analyzed by one-way analysis of variance (ANOVA) and t-test.
M. chamomilla was found to be similar to metformin as they both significantly increased PCOS-induced lowered levels of testosterone, LH, T3, T4, CAT, GPX. In contrast, in treated animals FSH, TSH, MDA were significantly decreased. Microscopic examination revealed restoration of normal follicular cells morphometry, histology and ultrastructure. Apoptosis based on caspase-3 expression was prevented by both treatments. In conclusion, PCOS in this study was demonstrated to alter both thyroid structure and function. M. chamomilla with its antioxidants contents markedly ameliorated such changes and could be advised as an adjuvant supplement for PCOS patients to protect against possible thyroid alteration. |
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Supervisor |
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Dr. Ahlam Abdulaziz Alahmadi |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1441 AH
2020 AD |
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Added Date |
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Tuesday, May 26, 2020 |
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Researchers
| أريج علي الزهراني | Alzahrani, Areej Ali | Researcher | Master | |
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