Document Details

Document Type : Thesis 
Document Title :
IDENTIFICATION OF THE CAUSATIVE MECHANISMS OF ALZHEIMER’S DISEASE
التعرف على آليات أسباب مرض الزهايمر
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : The goal of this study is to test key features of an alternative formulation of the amyloid hypothesis which postulates that intraneuronal accumulation of amyloid aggregates is a key event of pathogenesis. The essential problem for Alzheimer's disease (AD) is that the pathogenesis mechanism is not fully understood. According to the amyloid (Aβ) cascade hypothesis, is secreted from neurons and aggregates in the extracellular leading to neuronal dysfunction from the outside. However, recent findings taking the opposite direction, suggest that amyloid initially aggregates inside neurons, causing neuronal dysfunction and death and ultimately the release of the amyloid to the extracellular space. In addition, the analysis of Aβ from the deposits of Alzheimer's disease patients brain shown that Aβ aggregation begins within cells and consists of (Aβ42). The specific aims are to test whether the beta amyloid Aβ42 aggregation rise inside the neuron or not. In addition, to confirm the presence of the beta amyloid Aβ42 oligomeric for which prove to be present in the Alzheimer's patient's brain. The confocal microscopy technique indicate that the amyloid accumulation index increases over time and the number of neuronal plaques decreased within time. In addition, immunocytochemistry on the cells confirmed that there was significant increase in fluorescent spread indicating the amyloid beta oligomeric with the time increased. Moreover, the findings are supported in the cell viability (MTT assay) which determined decreased in cell survival rate with time frame. Then, it was confirmed that the presence of beta Aβ42 by Western blot. This study light up the hope in the way of drug discovery to be a significant benefit in the treatment of Alzheimer's disease and made critical test of the role of intraneuronal amyloid in the pathogenic mechanisms of AD and the foundation of the amyloid hypothesis. 
Supervisor : Prof. Taha Abdullah Kumosani 
Thesis Type : Doctorate Thesis 
Publishing Year : 1441 AH
2020 AD 
Added Date : Wednesday, June 17, 2020 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
شهد شاكر السنوسيAlsunusi, Shahad ShakerResearcherDoctorate 

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