Document Details

Document Type : Thesis 
Document Title :
POTENTIAL PROTECTIVE EFFECTS OF VORINOSTAT ON RENAL AND LIVER INJURY INDUCED BY CISPLATIN TREATMENT IN RATS.
التاثير الوقائى المحتمل لعقار الفورينوستات على اصابة الكلى و الكبد المحدثة بالعلاج بعقار السيبلاتين فى الجرذان.
 
Subject : Faculty of medicine 
Document Language : Arabic 
Abstract : Background and aim: Cisplatin "cis-diamminedichloroplatinum [II]; CDDP is the most widely used drug in cancer chemotherapy. The nephrotoxicity and hepatotoxicity of CDDP are two of its major side effects. Low dose vorinostat (LdVST) has been recognized to have antioxidant and anti-inflammatory efficacy both in vitro and in vivo models. The present study aimed to explore the potential protective effects low dose of VST against CDDP-induced kidney and liver toxicity in male Wistar rats. Methods: The rats were randomly divided into 4 groups (each 6 rats); I-control (5% DMSO (G.gav.) and Ns (IP)) group, II-CDDP (7.5 mg/kg IP in a single dose 5 days before the end of the experiment) group, III-VST (15 mg/kg/day (G. gav.) for 28 days) group, and IV-CDDP + VST group (as in group II & III). Blood, kidneys and livers were collected on day 28th for biochemical and histopathological examinations. Results: Administration of CDDP in a single dose produced a significant decrease in renal and hepatic tissue glutathione (GSH) levels and a significant increase in serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) liver enzymes, blood urea nitrogen (BUN), creatinine (SCr), renal and hepatic tissue malondialdehyde (MDA), tumor suppressor protein (p53), tumor necrosis factor-alpha (TNF-α) and nuclear factor kappa B (NF-κB) levels compared to control group. Pre-treatment with LdVST for 28 days significantly attenuated all unfavorable changes of these parameters. Histopathological analysis showed LdVST significantly decreased kidney and liver inflammatory induced by CDDP. LdVST also significantly increased Bcl-2 and decreased Caspas-3 immunoexpression in renal and hepatic tissues. In conclusion: These results suggested that LdVST alleviates CDDP-induced renal and hepatic toxicity in rats that might show a novel therapeutic potential for the management of these toxicities. 
Supervisor : Dr. Ahmed Shaker Ali 
Thesis Type : Master Thesis 
Publishing Year : 1441 AH
2020 AD 
Co-Supervisor : Prof. Hala Salah Abd El-Kawy Eweis 
Added Date : Monday, June 22, 2020 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
امنيه محمد بشرفBashraf, Omnyah MohammedResearcherMaster 

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