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Document Type |
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Thesis |
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Document Title |
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STUDY OF THE POTENTIAL USE OF BEE HONEY IN THE TREATMENT OF ATOPIC DERMATITIS IN HUMAN دراسة إمكانية استخدام عسل النحل فى علاج مرض الأكزيما في الإنسان |
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Subject |
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faculty of science |
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Document Language |
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Arabic |
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Abstract |
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The study aimed at the utility of Manuka honey (The name ‘Manuka’ is derived from the Maori language, the indigenous people of New Zealand) for 14 days to correct dysbiosis of skin microbiome of Saudi patients with Atopic Dermatitis (AD) or Eczema. Samples were collected from skin of patients meeting the inclusion/exclusion criteria after ethical approval has been awarded. A total of four male participants aged between 17- to 33-years old were recruited. Subjects manifested AD at different parts of their bodies (inner elbow, back knee, behind the ankle, and at the back) at the right and left sides. Treatment was applied on the right site, while the left site of the body was left untreated. DNAs from the two sides of different body parts were purified and V3-V4 region of bacterial 16S rRNA was amplified. Then, amplicons were shipped to Beijing Genome Institute (BGI) in China for deep sequencing and microbiomes were analysed. Phylogenetic tree indicated that the most common phyla are Actinobacteria, Bacteroidetes, Firmicutes, Fusobacteria and Proteobacteria. Statistical analysis for the highly abundant OTUs indicated that only five taxa showed significant differences due to Manuka honey application. Two of these taxa, e.g., Pseudomonas stutzeri and unassigned species of Micrococcus significantly increased due to treatment, while Staphylococcus aureus, unassigned species of Erwinia and Pseudomonas decreased due to treatment. Overall, the study indicated the influence of honey in decreasing abundance of Staphylococcus aureus, the causal microbe of AD, and in correcting the abundance of several other skin microbes. We speculate that non-virulent strain of S. aureus might interact with the virulent strain of which the first acts in blocking internalization and spreading of the virulent strain in epithelial cells by blocking abnormal T cell proliferation, thus, blocking the recovery of imbalanced Th1/Th2 ratio that result in the inability of the immune system to resist the disease. The other two high abundant taxa Pseudomonas stutzeri and unassigned species of Micrococcus might participate in the latter actions. Overall, the results provide new insights into the efficacy of analyzing microbiomes of patients with AD and the outcomes of applying honey to correct microbial dysbiosis in human. |
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Supervisor |
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Prof. Dr. Ahmed Bahieldin |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1441 AH
2020 AD |
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Co-Supervisor |
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Dr. Raed Saleh Albiheyri |
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Added Date |
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Monday, June 22, 2020 |
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Researchers
| لجين صالح طلبه | Tolbah, Lojayn Saleh | Researcher | Master | |
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