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Document Type |
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Thesis |
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Document Title |
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EVALUATION OF THE MARINE-DERIVED MICROBES AS A PROMISING SOURCE OF BIOACTIVE COMPOUNDS تقييم الميكروبات البحرية كمصدر واعد للمركبات الفعالة بيولوجيا |
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Subject |
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faculty of Pharmacy |
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Document Language |
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Arabic |
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Abstract |
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The use of marine microorganisms has been verified to be a reliable source of secondary metabolites that can be used for sustainable production of different bioactive compounds in large quantities. Associated/symbiotic marine fungi were isolated from different Red Sea marine invertebrates including sponges, tunicates, soft corals and sea slug. Small-scale cultures of the fungi were extracted with organic solvents and screened for their cytotoxic activities. The fungal extracts No. 20, 24 and 29 exhibited selective and strong activity against colorectal carcinoma (HCT 116) with inhibitory concentration (IC50) of 11.4-19 µg/mL. In addition, the fungal extract No. 8, which was isolated from a sponge exhibited selective and strong activity against breast cancer cell line (MCF7) with IC50 of 12.3 µg/mL. Further chemical investigations of the active extract No. 24 (CZY-103) of Fusarium sp. existed in tunicate Nephthea sp. in order to isolate potential compounds were carried out. A large-scale culture of the fungus was incubated in 30 °C for 14 days. The fungal broth was separated from mycelia by filtration, followed by extraction of the broth with organic solvent. Using different chromatographic techniques, four compounds were isolated from the EtOAc extract of the cultured Fusarium species. The structures of the isolated compounds were determined by using different techniques like co-chromatography and spectroscopic data including ESI-MS, 1H NMR, 13C NMR and 2D NMR including COSY, HSQC, HMBC and NOESY experiments. Compound 1 was assigned as 5Z-tricosenoic acid, compound 2 was identified as clionasterol, compound 3 was assigned as (2E,4E)-4-(methoxycarbonyl)-2-methylhexa 2,4-dienoic acid and compound 4 was assigned as (E)-7-hydroxy-7-(3-hydroxy-2,3-dimethylcyclopentyl)-3-methyloct-3-en-2-one. |
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Supervisor |
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Prof. Dr. Diaa Tohamy Ali Youssef |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1442 AH
2020 AD |
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Co-Supervisor |
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Prof. Dr. Dina M. Rafik Abou-hussein |
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Added Date |
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Tuesday, September 8, 2020 |
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Researchers
| سهى سعود رفه | Raffah, Suha Saud | Researcher | Master | |
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