Document Details

Document Type : Thesis 
Document Title :
SCREENING FOR COMMON AND RARE VARIANTS OF PROLACTIN GENE IN SAUDI WOMEN WITH UTERINE LEIOMYOMA.
فحص المتغيرات النادرة والشائعة في جين البرولاكتين في النساء السعوديات المصابين بورم الرحم الليفي
 
Subject : Faculty of medicine 
Document Language : Arabic 
Abstract : Uterine leiomyomas are highly prevalent benign smooth muscle tumors of the uterus seen in 70% of women. They are hormone responsive tumors and have been known to secrete prolactin (PRL), a hormone of the anterior pituitary gland. Elevated levels of serum Prolactin is a common clinical finding in uterine leiomyomas. However, the underlying causes for this elevation are not yet known. Therefore, the current study aims to measure the status of serum prolactin in UL patients and to investigate its molecular connection with coding region mutations of PRL gene in UL tissues. The serum prolactin levels in blood samples of UL patients were measured through the ELISA method. The coding region PRL gene mutations in UL and corresponding myometrium tissues were screened through Sanger sequencing. Results show that UL patients demonstrated elevated PRL serum levels. As a result, no somatic mutations in PRL gene have been identified. However, four germline variants (c.570G>A, c.205-102T>A, c.312+177T>C and c.269C>T) were detected. In conclusion, this study is the first to confirm serum prolactin level elevation in UL patients is not caused by somatic mutations in prolactin gene. However genetic polymorphisms may be indirectly contributing to the disease etiology. Hence, we recommend a larger case-control study to verify if PRL gene germline polymorphisms could act as potential genetic markers for the disease diagnosis, prognosis and/or its treatment. 
Supervisor : Prof. Nabeel Salem Bondagji 
Thesis Type : Master Thesis 
Publishing Year : 1442 AH
2020 AD 
Co-Supervisor : Dr. Ramu Elango 
Added Date : Sunday, January 17, 2021 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
حنان عبد الحليم السكريElSokary, Hanan AbdelhalimResearcherMaster 

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