Document Details
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Document Type |
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Thesis |
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Document Title |
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Potential Curative Effect of Vitamin D3 in Atopic Dermatitis in an Experimental Model in Mice التأثير العلاجي المحتمل لفيتامين د3 في التهاب الجلد التأتبي (الاكزيما) في نموذج تجريبي في الفئران |
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Subject |
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Faculty of medicine |
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Document Language |
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Arabic |
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Abstract |
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Atopic dermatitis (AD) is a common, relapsing, and long-lasting inflammatory skin disease. Its treatment often consumes both time and cost. Moreover, the current medications for AD are corticosteroids and non-corticosteroid immunosuppressive agents. However, the long-term use or abuse of these agents lead to several adverse effects. Vitamin D had been studied in various inflammatory diseases. Thus, the present study aimed to investigate the curative effect of vitamin D3 in ovalbumin (OVA)-induced AD mice versus dexamethasone and betamethasone for systemic and topical administration, respectively. AD was induced in BALB/c mice models using OVA, a chicken egg white protein. The current work was divided into two experimental parts: the systemic experiment in which vitamin D3 and dexamethasone were given IP and the topical experiment in which vitamin D3 cream and betamethasone cream were applied topically. The mice were divided in each experiment into five groups as follows: the normal control, OVA-induced AD, OVA-induced AD + vehicle, OVA-induced AD + vitamin D3 treated, and OVA-induced AD + corticosteroid treated groups. Moreover, the AD appearance was evaluated by the TIS score, the levels of IgE, Th2 cytokines (IL-5, 4, and 13), TNF-α, filaggrin levels, and epidermal thickness were measured. Also, a histopathological study was performed to confirm the anti-inflammatory effect of vitamin D3. Vitamin D3 observed a marked improvement in the treated mice skin according to the TIS score versus the OVA-induced AD group. Also, vitamin D3 showed a significant decrease (P < 0.05) in the IgE, IL-5, filaggrin, and epidermal thickness levels, and a significant increase (P < 0.05) in the IL-4 and -13 levels for both systemic and topically administered groups compared to the OVA-induced AD group. Furthermore, a non-significant difference was shown in most parameters for the vitamin D3 treated groups (both systemic and topically) versus the standard control drugs (corticosteroids). This was further confirmed by the histopathological study. Thus, it can be concluded that the present study has shed light on the importance of vitamin D3 (whether systemic or topically) for the treatment of AD, which may provide a promising future agent. |
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Supervisor |
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Prof. Mai Abdul Alim Abdul Sattar Ahmad |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1442 AH
2020 AD |
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Co-Supervisor |
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Dr. Huda Mohammed Alkreathy |
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Added Date |
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Sunday, February 28, 2021 |
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Researchers
| ندى سعود العصيمي | Alosaimi, Nada Saud | Researcher | Master | |
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