Document Details

Document Type : Thesis 
Document Title :
Effect of Curcumin on Ovarian Cancer Cells Growth
تأثير الكركمين على نمو خلايا سرطان المبيض
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : Ovarian cancer is the 7th cause of death from cancer in women worldwide and the second most common gynecological malignancy. There has been extensive and enough confirmations about the utilization of curcumin in cancer prevention due to its anti-proliferative and anti-carcinogenic properties or as an adjunct in general cancer treatment. In this study, the three main compounds of curcuminoids: curcumin, desmethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC) were determined by reversed-phase high- performance liquid chromatography (RP-HPLC). Afterward, the cytotoxicity of curcuminoids against ovarian cancer cell line (SKOV-3) was investigated. The obtained results showed that curcuminoids has been found to be highly cytotoxic to the ovarian cancer cells using a demonstrated concentration (30 µM). The cell viability assay showed that curcuminoids inhibited the cell growth of SKOV-3 cells in a dose (p < 0.05) and time-dependent (p < 0.05) way. Flow cytometry data revealed that curcuminoids significantly decreased cell populations in G1/G0, S, and G2/M phases at 24 h and 48 h after exposure to 30 µM of curcuminoids and led to apoptosis in a time-dependent manner (p<0.001). Most of the cells were found in sub-G1cells (apoptosis) when the cells were treated for 24 hours (p < 0.05). The apoptotic activity of curcuminoids showed an increase in the apoptotic cell population following incubation with curcuminoids compared with the negative controls by flow cytometry. Investigating the activity in SKOV-3 presents curcuminoids as a tumor suppressor in ovarian cancer. Furthermore, the wound-healing assay demonstrated that curcuminoids treatment remarkably suppressed the migration of SKOV-3 cells. The main obstacle preventing curcuminoids from being used clinically is their poor solubility in water and poor bioavailability by the oral route. Therefore, further studies are needed to support the potential and suitability of curcuminoids as a therapeutic agent and to evaluate the mechanisms of action to understand the apoptotic processes in ovarian cancer. 
Supervisor : Dr. Samar Abdullah Damiati 
Thesis Type : Master Thesis 
Publishing Year : 1442 AH
2020 AD 
Co-Supervisor : Dr. Mazin Abdulaziz Zamzami 
Added Date : Saturday, March 6, 2021 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
هبة صالح الموسىAlmousa, Heba SalehResearcherMaster 

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