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Document Type |
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Thesis |
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Document Title |
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Phytochemical and Biological Study of Euphorbia schimperi دراسة كيميائية وبيولوجية لنبات يوفوربيا شيمبيري |
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Subject |
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faculty of Pharmacy |
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Document Language |
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Arabic |
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Abstract |
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The aim of current study was to isolate and identify active compounds of Euphorbia schimperi C. Presl aerial parts (family Euphorbiaceae) and evaluated their cytotoxic activity on three different cell lines: MCF-7 (breast cancer), HepG2 (hepatocellular carcinoma), and HCT-116 (colon cancer). Phytochemical study of the methanolic extract of Euphorbia schimperi aerial parts using different chromatographic techniques led to the isolation of two new compounds: named cycloschimperol A (1) and cycloschimperol B (2), beside two known compounds: 24-methylenecycloartane (3) and cycloart-25-ene-3-one (4), The structures of the isolated compounds were established on the basis of physical, chemical and spectral data (IR, 1D (1H and 13C NMR) and 2D NMR (1H-1H COSY, HSQC, HMBC and NOESY), as well as HRESIMS and comparison with literature data. Their cytotoxic activity was assessed towards HepG2, MCF7, and HCT116 cancer cell lines by sulphorhodamine B test (SRB). It is noteworthy that compound) 2( and compound (4) demonstrated a potent activity towards HepG2, MCF7, and HCT116 cells with IC50s ranging from 1.4 to 4.7 μM compared to doxorubicin (IC50 0.18, 0.60, and 0.20 μM, respectively), whereas, compound (3) showed low cytotoxic potential with IC50s ranging from 19.7 to 55.6 μM. However, (1) did not show any activity up to a concentration 100 μM towards three tested cancer cell lines. |
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Supervisor |
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Dr. Jamal Hussein |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1442 AH
2020 AD |
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Added Date |
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Saturday, March 6, 2021 |
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Researchers
| محمد فؤاد بنجر | Banjar, Mohammed Fouad | Researcher | Master | |
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