Document Details

Document Type : Thesis 
Document Title :
SEROLOGICAL AND MOLECULAR STUDIES OF BRUCELLOSIS IN IMPORTED ANIMALS, SAUDI ARABIA
دراسات مصلية وجزيئية لمرض البروسيلا للمواشي المستوردة بالمملكة العربية السعودية
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : ABSTRACT Brucellosis is a transmissible bacterial zoonotic disease in animals as well as humans. It is caused by Gram-negative coccobacillus bacteria of the genus Brucella. Sera and blood samples were collected from imported animals (sheep, goats, and camels) from different countries Sudan, Jupiter, and Georgia from May 2017 to July 2018. A total of 4457 sera were subjected to Rose Bengal Test (RBT) and confirmed with competitive ELISA (ELISA) and Insulated Isothermal PCR (iiPCR). The prevalence was 0.71% and the highest prevalence (2.8 %) and the lowest prevalence (0.4 %) reported in camels and sheep respectively from Sudan. According to the origin of Animals, Jupiter reported the highest prevalence 1.8 % and the lowest prevalence was reported in animals imported from Sudan. According to the origin of Animals, Jupiter reported the highest prevalence 1.8 % and the lowest prevalence was reported in animals imported from Sudan. On the other hand, according to an animal’s species, the highest prevalence (1.95 %) was reported in camels followed by (0.84 %) in goats, and the lowest prevalence (0.56 %) was reported in sheep. Out of 57 sera, samples tested by ELISA (which included 32 the positive samples by RBT) only 30 samples were positive with a prevalence value (52.6 %) False-positive results of RBT marked in 7 samples out of 32 samples negative to cELISA. Brucellosis seroprevalence was observed in imported Animals from different countries during this study. Two Brucella species were detected by iiPCR they were; Brucella abortus from blood and Brucella melitensis from the milk of a goat. A mutual collaboration may be required to develop an appropriate serodiagnosis and molecular technique; the field-deployable iiPCR platform has the potential to aid in the rapid detection of a variety of animal and human infections under field conditions and it may be applied to all species of Brucella. 
Supervisor : Dr. Mohamed Ahmed 
Thesis Type : Master Thesis 
Publishing Year : 1442 AH
2021 AD 
Added Date : Wednesday, May 26, 2021 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
سليمان محمد ابوسليمانAbu Sulaiman, Suleiman MohammedResearcherMaster 

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