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Document Type |
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Thesis |
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Document Title |
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PROTEOMICS ANALYSIS OF MILK FAT GLOBULAR MEMBRANE PROTEINS OF SAUDI (HAMRAE, AND MAGAHEEM) CAMELUS DROMEDARY CLANS. تحليل محتوي البروتين لعضيات حليب عشائر (الحمر والمجاهيم) الابل السعودية |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Milk fat globule membrane (MFGM) proteins have several benefits for infants. Our research studied and analysed the MFGM proteins for two of the Saudi camel clans Camelus dromedarius (C. dromedarius) (Magaheem and Hammrae ) throughout the lactation cycle, as well as the heterogeneity of MFGM proteins at the interclan and intraclan levels. The sampling phase was carried out in four sets during the four seasons of the year at different locations in Jeddah city, where 105 samples were collected in total. MFGM protein ingredients were extracted and estimated by protein analysis method “Bradford protein analysis”. The protein profiles of MFGM and the differences in the interclan and intraclan levels were studied and investigated using SDS-PAGE, SDS-UREA-PAGE and Proteomic analysis. The presence of some key proteins in camel MFGM, was also determined using ELISA analysis. Finally, the presence of some main camel MFGM proteins was documented such as: butyrophillin (Btn), Lactadherin (LDH), Vinculin, Lactoferrin (LF), Acetylserotonin O-methyltransferase, Septin-6, Casein kinase CK, Actin, Mucin (MUC), Fatty acid-binding protein (FABP) and CK1 that are present in different concentrations depends on different camels, clans and seasons. Moreover, results of MFGM proteins analysis showed that there were variance protein concentrations for Magaheem camel clan compared to Hammrae . Our results clearly revealed that there are multiple differences in the interclans and intraclans levels in the protein profiles as detected in SDS-PAGE. Proteomic analysis confirmed that outcome, and extended our knowledge that there are major, minor and redundant proteins spots. |
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Supervisor |
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Dr. Hussien Almehdar |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1442 AH
2021 AD |
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Added Date |
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Saturday, June 5, 2021 |
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Researchers
| فؤاد خلف الزهراني | Alzahrani, Fuad Khalaf | Researcher | Master | |
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